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Team:NCTU Formosa/Notebook/test5
From 2014.igem.org
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<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 8/11 | + | 8/11</p> |
| - | + | ||
| - | + | ||
| - | </p> | + | |
<p> | <p> | ||
| - | + | Transformation of NCTU1 (G1)</p> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | </p> | + | |
<p> | <p> | ||
| - | 8/13 | + | Transformation of PBAN (G2)</p> |
| - | Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) | + | <p> |
| - | Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) | + | 8/12</p> |
| - | Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2) | + | <p> |
| - | </p> | + | Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p> |
| + | <p> | ||
| + | Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p> | ||
| + | <p> | ||
| + | Mini-prep of [Pcons]Ar</p> | ||
| + | <p> | ||
| + | Digestion of [Pcons]Ar (ES) (G2)</p> | ||
| + | <p> | ||
| + | 8/13</p> | ||
| + | <p> | ||
| + | Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p> | ||
| + | <p> | ||
| + | Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p> | ||
| + | <p> | ||
| + | Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)</p> | ||
<p> | <p> | ||
| - | 8/14 | + | 8/14</p> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | </p> | + | |
<p> | <p> | ||
| - | 8/15 | + | Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p> |
| - | Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) | + | <p> |
| - | Purification from electrophoresis:[PBAN]No.4&5&6 | + | Purification from electrophoresis:[PBAN]No.4&5&6(G1)</p> |
| - | Transformation of [B0034]Ar (G2) | + | <p> |
| - | </p> | + | Transformation of the ligation product made yesterday</p> |
| + | <p> | ||
| + | Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)</p> | ||
| + | <p> | ||
| + | 8/15</p> | ||
| + | <p> | ||
| + | Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p> | ||
| + | <p> | ||
| + | Purification from electrophoresis:[PBAN]No.4&5&6</p> | ||
| + | <p> | ||
| + | Transformation of [B0034]Ar (G2)</p> | ||
<p> | <p> | ||
| - | 8/16 | + | 8/16</p> |
| - | Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) | + | <p> |
| - | PCR:[B0034] | + | Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1)</p> |
| - | Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034 | + | <p> |
| - | Mini-prep of [B0034]Ar E.coli | + | PCR:[B0034]</p> |
| - | Ligation:[Pcons]+[B0034] +PSB1K3 | + | <p> |
| - | Digestion of [BFP](ES) | + | Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034</p> |
| + | <p> | ||
| + | Mini-prep of [B0034]Ar E.coli</p> | ||
| + | <p> | ||
| + | Ligation:[Pcons]+[B0034] +PSB1K3</p> | ||
| + | <p> | ||
| + | Digestion of [BFP](ES)</p> | ||
| + | <p> | ||
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])</p> | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])</p> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 8/17 | + | 8/17</p> |
| + | <p> | ||
Electrophoresis of the digestion products:[PBAN]No.4&5&6 (EP) (G1)</p> | Electrophoresis of the digestion products:[PBAN]No.4&5&6 (EP) (G1)</p> | ||
<br></div> | <br></div> | ||
Revision as of 14:17, 13 October 2014
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8/11
Transformation of NCTU1 (G1)
Transformation of PBAN (G2)
8/12
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr
Mini-prep of [Pcons]Ar
Digestion of [Pcons]Ar (ES) (G2)
8/13
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)
8/14
Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
Purification from electrophoresis:[PBAN]No.4&5&6(G1)
Transformation of the ligation product made yesterday
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Purification from electrophoresis:[PBAN]No.4&5&6
Transformation of [B0034]Ar (G2)
8/16
Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1)
PCR:[B0034]
Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034
Mini-prep of [B0034]Ar E.coli
Ligation:[Pcons]+[B0034] +PSB1K3
Digestion of [BFP](ES)
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])
8/17
Electrophoresis of the digestion products:[PBAN]No.4&5&6 (EP) (G1)
