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| Line 68: |
Line 68: |
| | <div class="calendar-note"> | | <div class="calendar-note"> |
| | <div class="single note"> | | <div class="single note"> |
| - | <p>7/1
| + | 1 |
| - | Ligation of [J23101+B0032+PSB1C3] Cr (G1)
| + | |
| - | Digestion of [B0032] Ar
| + | |
| - | Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
| + | |
| - | </p>
| + | |
| - | <p>7/2
| + | |
| - | Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1)
| + | |
| - | Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)
| + | |
| - | </p><br></div>
| + | |
| - | <div class="single note">
| + | |
| - | <p>7/3
| + | |
| - | Mini-prep of [B0032+BFP+J61048] and then digestion: (XP)
| + | |
| - | Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 7/4
| + | |
| - | Digestion of [T7 promoter](SP) (G2)
| + | |
| - | Cultivation of [J23101] Ar (G3)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 7/5
| + | |
| - | PCR:[B0032+K592100+J61048*11] Ar (G1)
| + | |
| - | Electrophoresis of PCR products (G1)
| + | |
| - | Ligation of [J23101+B0032+PSB1C3*3] Cr (G1)
| + | |
| - | Electrophoresis of plasmid and digestion products:[T7promoter](SP),[B0031+BFP+J61048](XP)
| + | |
| - | Ligation:[T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)
| + | |
| - | </p><p>7/6
| + | |
| - | Transform the ligation products made at 7/5 (G2)
| + | |
| - | </p><p>
| + | |
| - | 7/7
| + | |
| - | Transformation of [J23101+B0032*5] Cr (G1)
| + | |
| - | Cultivation of [B0032+K592100+J61048*2] Ar (G1)
| + | |
| - | Cultivation of [J23101] Ar (G3)
| + | |
| - | Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)
| + | |
| - | </p><p>
| + | |
| - | 7/8
| + | |
| - | Mini-prep of [B0032+K592100+J61048*2] Ar and then digest only one of two: (XP) (G1)
| + | |
| - | PCR:[T7+B0032+BFP+J61048] (G2)
| + | |
| - | Digestion of [J23101] Ar (ES) (G3)
| + | |
| - | </p><p>
| + | |
| - | 7/9
| + | |
| - | Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)
| + | |
| - | </p><p>
| + | |
| | <br></div> | | <br></div> |
| | <div class="single note"> | | <div class="single note"> |
| - | <p>
| + | 2 |
| - | | + | |
| - | | + | |
| - | | + | |
| | <br></div> | | <br></div> |
| | <div class="single note"> | | <div class="single note"> |
| - | <p>
| + | 3 |
| - | 5/19
| + | |
| - | Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)
| + | |
| - | </p><p>
| + | |
| - | 5/20
| + | |
| - | Transform of [T7polymerase] Kr and [PSB1K3] Kr (G2)
| + | |
| - | Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)
| + | |
| - | Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)
| + | |
| - | Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 5/21
| + | |
| - | Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)
| + | |
| - | Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2)
| + | |
| - | Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)
| + | |
| - | Ligation of [K592100+J61048+PSB1K3] Kr (G1)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 5/22
| + | |
| - | Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 5/23
| + | |
| - | Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 5/24
| + | |
| - | Digestion of [J61048] Ar (XP) (G2)
| + | |
| - | PCR: [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)
| + | |
| - | Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)
| + | |
| - | </p>
| + | |
| - | | + | |
| | <br></div> | | <br></div> |
| | <div class="single note"> | | <div class="single note"> |
| - | <p>
| + | 4 |
| - | 5/27
| + | |
| - | Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
| + | |
| - | Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2)
| + | |
| - | Cultivation of [PSB1K3*3] Kr(G2)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 5/28
| + | |
| - | PCR:[PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1)
| + | |
| - | Mini-prep of [PSB1K3*3] Kr(G2)
| + | |
| - | Digestion of [PSB1K3*3] Kr (EP) (G2)
| + | |
| - | Electrophoresis of plasmid and digested [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | | + | |
| - | | + | |
| - | 5/29
| + | |
| - | Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
| + | |
| - | Transform of [J23101] Ar (G1)
| + | |
| - | Transform of [T7 promoter] Ar Kr (G2)
| + | |
| - | Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2)
| + | |
| - | Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2)
| + | |
| - | Ligation of [Plac+B0032+PSB1K3] Kr (G3)
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | 5/30
| + | |
| - | Transform of ligation product (G2)
| + | |
| - | Transform of ligation product [Plac+B0032+PSB1K3] Kr (G3)
| + | |
| - | </p>
| + | |
| | <br></div> | | <br></div> |
| | </div> | | </div> |
| | <div class="clear"></div> | | <div class="clear"></div> |
| | </div> | | </div> |
"
