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Team:Goettingen/proLP
From 2014.igem.org
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4> | ||
<ul> | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>PfuS</i> PCR</a></li> | ||
| + | |||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Fusi_PCR">Fusion PCR</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Fusi_PCR">Fusion PCR</a></li> | ||
| - | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#E_col"><i>E.coli</i> colony PCR</a></li> | |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Y_col">Yeast colony PCR</a></li> |
</ul> | </ul> | ||
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<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | ||
| - | + | ||
| - | + | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Y2H Assay with Robot</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Y2H Assay with Robot</a></li> | ||
Revision as of 11:52, 10 October 2014
Overview
PCR Methods
DNA Assessment
Plasmid Construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
