Team:ETH Zurich/labblog/20140825meet
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==== Monday, August 25th ==== | ==== Monday, August 25th ==== | ||
- | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check <html> <a | + | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check <html> <a id="insidelink" target="cross-talk" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. |
*We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. | *We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. |
Revision as of 23:03, 7 October 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check the corresponding article for more details.
- We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.