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Team:Austin Texas/interlab study
From 2014.igem.org
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| - | Add | + | =Devices Measured= |
| + | *BBa_J23101 (constitive promoter) + BBa_E0240 (B0032 - RBS, E0040 - GFP, B0015 - double terminator) in pSB1C3 | ||
| + | *BBa_J23101 (constitive promoter) + BBa_E0240 (B0032 - RBS, E0040 - GFP, B0015 - double terminator) in pSBK3 | ||
| + | *BBa_J23115 (constitive promoter) + BBa_E0240 (B0032 - RBS, E0040 - GFP, B0015 - double terminator) in pSB1C3 | ||
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| + | =Protocols= | ||
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| + | ==Sample Preparation== | ||
| + | *Inoculate 10 mL of LB with frozen stocks of the three transformed clones (I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 electrocompetent cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm. | ||
| + | *The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. | ||
| + | *Add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | ||
| + | |||
| + | ==Measurement Protocol== | ||
| + | *The samples were plated on a 96-well plate as follows: | ||
| + | * | ||
| + | *The 96-well plate was inserted into the Infinite 200 PRO Microplate Reader | ||
| + | *Open Tecan i-control, the software used to document the readings | ||
| + | *Configure the settings as detailed above | ||
| + | *Obtain fluorescence and OD600 readings | ||
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| + | =Sequencing Data= | ||
Revision as of 04:31, 10 October 2014
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