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Team:UFAM Brazil/8-8-2014
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Revision as of 19:30, 7 October 2014
08/08/2014 | ||
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Heeeyy !! Today remade the plasmid extraction with kit JM110, and DH5α with Biobrick for bioremediation and essential Biobrick. | ||
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Eletrophoretical profile of samples | ||
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1. Biobrick essencial extraído de DH5α (1) 2. Biobrick essencial extraído de DH5α (2) 3. Biobrick para biorremediação extraído de JM110 (1) 4. Biobrick para biorremediação extraído de JM110 (2) Após a extração, digerimos as amostras com EcoRI e PstI afim de analisar o tamanho do fragmento do Biobrick essencial e Biobrick para biorremediação. Utilizamos o seguinte sistema para digestão enzimática: | ||
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The digestion lasted 2 hours at 37°C. We applied the gel with 0.8% of agarose the total volume of each sample. Eletrophoretical profile of the digested samples: | ||
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1. Bioremediation Biobrick not digested. 2. Bioremediation Biobrick digested with EcoRI and PstI 3. Essential Biobrick not digested. 4. Essential Biobrick digested with EcoRI and PstI. 5-6-7-8. Plasmids did not show a good extraction Hurray!!! The digestion has released fragments that proved the viability our beloved biobricks. Bioremediation Biobrick has 1.823 base pairs and Essential Biobrickhas 1.234 base pairs,that’s why BB_Biorremediation sits above BB_Essential in the gel. =) We have also analyzed samples 5-6-7-8 that didn’t show digestion or it showed partial digestion (Either no presented digestion or not presented partial digestion). | ||
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