Team:TCU Taiwan/Notebook

experimental part:

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α.

other part:

experimental part:

other part:

Get foundation from nearby store (yeah!!!!!)
Decided to have iGEM contest in local school as human practice

Others don’t think E.Maat is a good name, under re-consideration

experimental part:

Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011

Prepare Cm. plate、LB. plate

other part:

Something wrong with gRNA for AmpR, redesign

Second project theme came out by Denise and Regina: Trogene horse. Needs later discuss to decided which one as theme( E.Maat or Trogene Horse)

Try to get foundation from Biotechnology company on e-mail.

Prepare for igem conference in NCTU-Formosa (ppt)

experimental part:

Incubate E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

Extract pSB1C3 BBa_I13521、BBa_ K914003、BBa_ K1218011

other part:

Final Theme decided: Trogene Horse

Prepare for igem conference in NCTU-Formosa (ppt)

Designing team logo and project logo

House started working on wiki

Decided to have a synthetic biology contest in National Hualien Girls’ Senior High School

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

other part:

Prepare for igem conference in NCTU-Formosa (ppt)

Designing team logo and project logo

House working on wiki construction

Prepare for the contest in National Hualien Girls’ Senior High School (ppt)

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

other part:

Prepare for igem conference in NCTU-Formosa (ppt).

Have a synthetic biology contest in National Hualien Girls’ Senior High School.

Order primer for gRNA team logo and project logo confirmed.

Attending iGEM conference in NCTU-Formosa

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate

other part:

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

Purification of RFP sequence from pSB1C3.

experimental part:

Ligation of RFP and pBluescript SK(-)

Transformation clone RFP/pBluescript SK(-) to DH5α

Extract plasmid clone RFP/pBluescript SK(-)

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.

other part:

Design badge as small gift

Order Helper phage for pBluescript SK(-)

Make movie for project introduction of Trogene horse.

Prepare iGEM presentation ppt、poster.

experimental part:

Incubate:

1. E.coli DH5α with BBa_I13521、(in pSB1C3 )

2. E.coli top10 with pBluescript II SK (-)

Extract :

1. pSB1C3: BBa_I13521

2. pBluescript

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted

Purification of RFP sequence from pSB1C3

Ligation Clone RFP/pBluescript SK(-)to DH5α Store BBa_I13521、BBa_ K914003、BBa_ K1218011、clone RFP/pBluescript SK(-)

other part:

Make movie for project introduction of Trogene.

horse Prepare iGEM presentation ppt、poster.

experimental part:

Ligation Clone RFP/pBluescript SK(-)

Extract plasmid clone RFP/pBluescript SK(-)

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted

Modeling for phage injection (Thanks for NCTU-Formosa’s help)

other part:

experimental part:

Ligation Clone RFP/pBluescript SK(-)

Extractplasmid clone RFP/pBluescript SK(-)

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted

Prepare competent cell(JM101)

incubate:

1. E.coli DH5α with BBa_I13521(pBluescript)、BBa_K1473001~3003(in pSB1C3 )

Extract :

1. pSB1C3: BBa_I13521、BBa_K1473001~3003

Enzyme digestion(PstI、EcoRI) ，確認抽plasmid的成果

PCR: tracrRNA、Cas 9+crRNA

Modeling for phage injection

other part:

Prepare iGEM presentation ppt、poster

Wiki construction

experimental part:

Ligation gRNA/ pSB1C3

Transformation gRNA/ pSB1C3 to DH5α

Incubate gRNA/ pSB1C3 in E.coli JM101

Extract plasmid gRNA/ pSB1C3 in E.coli JM101

Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted

PCR trRNA、Cas 9+crRNA

Prepare modeling

3 new biobrick finished

other part:

Have a iGEM contest(HP) in Tzu chi university

Prepare presentation ppt、poster

Wiki construction

other part:

Prepare iGEM presentation ppt、poster

Wiki construction