"
Team:ZJU-China/Processing
From 2014.igem.org
| Line 9: | Line 9: | ||
<p class="cutline"> </p> | <p class="cutline"> </p> | ||
<div class="zju_sec"> | <div class="zju_sec"> | ||
| - | |||
| - | |||
| + | <p>Put content here!</p> | ||
| + | <table class="img" style="float:none;width:100%"> | ||
| + | <tr><th>Details in Socket E.coli</th><th>Tasks for you</th></tr> | ||
| + | <tr><td></td><td><p>Design circuit and find your parts DNA</p></td></tr> | ||
| + | <tr><td></td><td><p>Use GS-BOX to design the strategy of circuit construction</p></td></tr> | ||
| + | <tr><td></td><td><p>PCR to add Homeoregion, Ligase standard parts.</p></td></tr> | ||
| + | <tr><td><p>Lambda red protein is expressed during cultivation</p></td><td><p>Making electrotranformation competent cells, use reporter 1 to select.</p></td></tr> | ||
| + | <tr><td><p>Inserted circuit(dsDNA) are introduced into Socket.coli.</p></td><td><p>electrotranformation</p></td></tr> | ||
| + | <tr><td><p>Inserted circuit recombine biterminator unit | ||
| + | rep-Int-exp.gif</p></td><td rowspan="2"><p>Cell recovery after electrotranformation, liquid cultivation.</p></td></tr> | ||
| + | <tr><td><p>Int express after recombination, flip over attB/attP site</p></td></tr> | ||
| + | <tr><td><p>Stage switched, reporter 2 expressed | ||
| + | int-flip-bp.gif | ||
| + | </p></td><td><p>plate cultivation, Use reporter 2 to select candidate cells</p></td></tr> | ||
| + | <tr><td><p>Xis express, Int+Xis will flip over attL/attR sites. Stage switched again and inverted biterminator unit flipped over to silence Int expression | ||
| + | int+xis-flip lr.gif | ||
| + | int+xis-flip T.gif | ||
| + | </p></td><td><p><b>select positive colony to amplify, Arabinose induce at the main time. Use plasmid backbone resistance to select.</b></p></td></tr> | ||
| + | <tr><td></td><td><p>plate cultivation, Use reporter 1 to select candidate cells. This survival cells are ready for the next recombination round.</p></td></tr> | ||
| + | <tr><td></td><td><p>If circuit construction is over, cultivate cell in 42℃ to discard Support device.</p></td></tr> | ||
| + | </table> | ||
</div> | </div> | ||
<p class="cutline"> </p> | <p class="cutline"> </p> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 17:27, 16 October 2014
| Team Sponsor | Contact us | ||
|---|---|---|---|
|
|
Team Forum(BBS): | ZJU-China 2014 Team Forum on www.zjubiolab.zju.edu.cn | |
| Post Address: | Biolab Center Room 413, ZJU Zijin'gang Campus, Yuhang Tang Road No.866, Hangzhou, Zhejiang |
||
| Powered by Media Wiki. Zhejiang University. | Email Address: | zjuchina2014 @ 163.com | |
Put content here!
| Details in Socket E.coli | Tasks for you |
|---|---|
Design circuit and find your parts DNA | |
Use GS-BOX to design the strategy of circuit construction | |
PCR to add Homeoregion, Ligase standard parts. | |
Lambda red protein is expressed during cultivation | Making electrotranformation competent cells, use reporter 1 to select. |
Inserted circuit(dsDNA) are introduced into Socket.coli. | electrotranformation |
Inserted circuit recombine biterminator unit rep-Int-exp.gif | Cell recovery after electrotranformation, liquid cultivation. |
Int express after recombination, flip over attB/attP site | |
Stage switched, reporter 2 expressed int-flip-bp.gif | plate cultivation, Use reporter 2 to select candidate cells |
Xis express, Int+Xis will flip over attL/attR sites. Stage switched again and inverted biterminator unit flipped over to silence Int expression int+xis-flip lr.gif int+xis-flip T.gif | select positive colony to amplify, Arabinose induce at the main time. Use plasmid backbone resistance to select. |
plate cultivation, Use reporter 1 to select candidate cells. This survival cells are ready for the next recombination round. | |
If circuit construction is over, cultivate cell in 42℃ to discard Support device. |
