Team:TCU Taiwan/Notebook

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(Difference between revisions)
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           Transformation ( Cas9 in pSB1C3 into DH5α)<br>Incubate DH5α with pSB1C3</font></p>
           Transformation ( Cas9 in pSB1C3 into DH5α)<br>Incubate DH5α with pSB1C3</font></p>
           <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font>          </p>
           <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font>          </p>
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           <p><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font><br><font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>Practice presentation<br>Design gRNA for AmpR<br>
+
           <p><h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5><br><font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>Practice presentation<br>Design gRNA for AmpR<br>
           Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
           Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br>
           Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font></p>
           Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font></p>

Revision as of 13:56, 7 October 2014

 
Notebook
  

Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

   
Week 1 6/22~6/28

experimental part:

Prepare for the competent cell

BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3

Extract plasmid with pSB1C3 from DH5α

other part:

Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)

Week 2 6/29~7/5
Test
Week 3 7/6~7/12
Test
Week 4 7/13~7/19
Test
Week 5 7/20~7/26
Test
Week 6 7/27~8/2
Test
Week 7 8/3~8/7
Test
Week 8 8/10~16
Test
Week 9 8/17~8/23
Test
Week 10 8/24~8/30
Test
Week 11 8/31~9/6
Test
Week 12 9/7~9/13
Test
Week 13 9/14~9/20
Test
Week 14 9/21~9/27
Test
Week 15 9/28~10/4
Test
^
    

^

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