Team:Paris Bettencourt/Project/TMAU

From 2014.igem.org

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<p class=text2><img src='https://static.igem.org/mediawiki/2014/b/b3/Sch%C3%A9ma_fish_odor_syndrom.jpg'></p>
<h6>Introduction</h6><br>
<h6>Introduction</h6><br>
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<p>Trimethylamine (TMA) is produced in the intestine by <i>Desulfovibrio desulfuricans</i> thanks to the fermentation of choline. In healthy patients, the FMO3 gene allows the degradation of TMA in the liver into a non-volatile compound, TMA oxide. But a mutation in the FMO3 sequence is most of the time the cause of TMAU: TMA is not degraded and is then excreted in sweat, saliva and urine leading to a strong fish odor. The patients are otherwise healthy but the disease affect their social relationships and can lead to depression. There is no cure for this metabolic disorder but some treatments, often based on avoiding some sorts of food, tend to lower the symptoms.</p>
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<p class=text1>Trimethylamine (TMA) is produced in the intestine by <i>Desulfovibrio desulfuricans</i> thanks to the fermentation of choline. In healthy patients, the FMO3 gene allows the degradation of TMA in the liver into a non-volatile compound, TMA oxide. But a mutation in the FMO3 sequence is most of the time the cause of TMAU: TMA is not degraded and is then excreted in sweat, saliva and urine leading to a strong fish odor. The patients are otherwise healthy but the disease affect their social relationships and can lead to depression. There is no cure for this metabolic disorder but some treatments, often based on avoiding some sorts of food, tend to lower the symptoms</p>
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<h6>Aims</h6><br>
<h6>Aims</h6><br>
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<p>The strain <i>Ruegeria pomeroyi</i>, a genus of the Rhodobacteraceae, produces an enzyme called trimethylamine monooxygenase thanks to the <i>tmm</i> gene. As FMO3, this enzyme degrades trimethylamine into trimethylamine-N-oxide but is adapted to a bacterial expression. The project aims at cloning <i>tmm</i> into <i>E.coli</i> and then into <i>Corynebacterium striatum</i>, one of the most common bacteria of the skin. The new strain would be integrated to the skin microbiome and would suppress the fish odor.</p>
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<p class=text1>The strain <i>Ruegeria pomeroyi</i>, a genus of the Rhodobacteraceae, produces an enzyme called trimethylamine monooxygenase thanks to the <i>tmm</i> gene. As FMO3, this enzyme degrades trimethylamine into trimethylamine-N-oxide but is adapted to a bacterial expression. The project aims at cloning <i>tmm</i> into <i>E.coli</i> and then into <i>Corynebacterium striatum</i>, one of the most common bacteria of the skin. The new strain would be integrated to the skin microbiome and would suppress the fish odor.</p>
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<h6>Results</h6><br>
<h6>Results</h6><br>
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Revision as of 16:33, 6 October 2014

BACKGROUND

Trimethylaminuria (TMAU) commonly called Fish Odor Syndrom, is a rare genetic disease; trimethylamine (TMA) is naturally produces in the gut by the intestinal flora but it supposed to be degraded in the liver by the FMO3 enzyme coded by a gene with the same name. The suffering patients have a mutated FMO3 gene, and TMA is excreted in sweat, saliva and urine, causing a strong fish odor.

AIMS

Tmm (trimethylamine monooxygenase) is a gene found in the bacteria Ruegeria pomeroyi and it is similar to the human FMO3 gene. The aim of this project is to clone tmm into Corynebacterium striatum, a bacteria commonly found in the skin. This strain would be incorporated in a cosmetic cream in order to remove the fish odor in trimethylaminuria patients.

 RESULTS

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BIOBRICKS

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Introduction Aims Part3 Part4 Part5

Introduction

Trimethylamine (TMA) is produced in the intestine by Desulfovibrio desulfuricans thanks to the fermentation of choline. In healthy patients, the FMO3 gene allows the degradation of TMA in the liver into a non-volatile compound, TMA oxide. But a mutation in the FMO3 sequence is most of the time the cause of TMAU: TMA is not degraded and is then excreted in sweat, saliva and urine leading to a strong fish odor. The patients are otherwise healthy but the disease affect their social relationships and can lead to depression. There is no cure for this metabolic disorder but some treatments, often based on avoiding some sorts of food, tend to lower the symptoms

Aims

The strain Ruegeria pomeroyi, a genus of the Rhodobacteraceae, produces an enzyme called trimethylamine monooxygenase thanks to the tmm gene. As FMO3, this enzyme degrades trimethylamine into trimethylamine-N-oxide but is adapted to a bacterial expression. The project aims at cloning tmm into E.coli and then into Corynebacterium striatum, one of the most common bacteria of the skin. The new strain would be integrated to the skin microbiome and would suppress the fish odor.

Results

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo.

Motivation

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Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com
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