Team:EPF Lausanne/Parts

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Revision as of 09:50, 5 October 2014

PARTS

Parts submitted by the 2014 EPFL iGEM team

Our team submitted a total of 43 Biobricks.

Biobrick What it is Function Why do we use it?
BBa_K1486000 CpxR coding sequence Transcription factor To make most of our biobricks!
BBa_K1486001 CpxR under arabinose promoter Treanscription factor
BBa_K1486002 PAra + sfGFP CpxR Expresses fused protein Test CpxR expression & Ara promoter
BBa_K1486003 Flexible linker Attaches two proteins together
BBa_K1486004 Flexible linker Attaches two proteins together
BBa_K1486005 PAra + CpxR sfGFP Expresses fused protein Test CpxR expression & Ara promoter
BBa_K1486006 IFP[1] N terminus of split IFP
BBa_K1486007 IFP[2] C terminus of split IFP
BBa_K1486008 CxpR & Split IFP1.4 [Cterm + Cterm] Two CpxR CDS, each C terminus attached to a moiety of IFP Characterize CpxR dimerization
BBa_K1486009 CxpR & Split IFP1.4 [Nterm + Nterm] Two CpxR CDS, each N terminus attached to a moiety of IFP Characterize CpxR dimerization
BBa_K1486010 CxpR & Split IFP1.4 [Nterm + Cterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization
BBa_K1486011 CxpR & Split IFP1.4 [Cterm + Nterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization
BBa_K1486012 CpxR + Nterm IFP CpxR with the Nterm moiety of IFP attached at its C terminus Intermediate & control
BBa_K1486013 CpxR + Cterm IFP CpxR with the Cterm moiety of IFP attached at its C terminus Intermediate & control
BBa_K1486014 Nterm IFP + CpxR CpxR with the Nterm moiety of IFP attached at its N terminus Intermediate & control
BBa_K1486015 Cterm IFP + CpxR CpxR with the Cterm moiety of IFP attached at its N terminus Intermediate & control
BBa_K1486016 Nterm fLuc N terminus moiety of the firefly luciferase
BBa_K1486017 Cterm fLuc C terminus moiety of the firefly luciferase
BBa_K1486018 PAra + Nterm fLuc + Cterm fLuc Split firefly luciferase under arabinose promoter Control
BBa_K1486019 Nterm rLuc C terminus moiety of the renilla luciferase
BBa_K1486020 Cterm rLuc N terminus moiety of the renilla luciferase
BBa_K1486021 PAra + Nterm rLuc + Cterm rLuc Split renilla luciferase under arabinose promoter Control
BBa_K1486022 rLuc Full renilla luciferase Control
BBa_K1486023 Yeast sfGFP Superfolder GFP for yeast cells Reporter
BBa_K1486024 Kan Yeast kanamycin resistance gene Selection marker
BBa_K1486025 ADH1 terminator Terminator
BBa_K1486026 Yeast sfGFP + Kan Yeast sfGFP attached to the yeast kanamycin resistance gene Control the expression of pbs2
BBa_K1486027 rLuc + Kan Renilla luciferase attached to the yeast kanamycin resistance gene
BBa_K1486028 Nterm Yeast sfGFP N terminal moiety of split yeast sfGFP
BBa_K1486029 Nterm sfGFP + kan Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene
BBa_K1486030 Nterm rLuc + kan Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene
BBa_K1486031 Ura CDS for Uracil (yeast selective purposes) Confer resistance to Uracil-deprived medium
BBa_K1486032 Yeast sfGFP + Ura Yeast sfGFP attached to the Uracil CDS Control the expression of hog1
BBa_K1486033 rLuc + Ura Renilla luciferase attached to the Uracil CDS Control the expression of hog1
BBa_K1486034 Cterm yeast sfGFP C terminal moiety of split the yeast sfGFP
BBa_K1486035 Cterm yeast sfGFP + Ura Cterm moiety of split yeast sfGFP attached to the Uracil CDS
BBa_K1486036 Cterm rLuc + Ura Cterm moiety of split renilla luciferase attached to the Uracil CDS
BBa_K1486037 linker Attaches two proteins together
BBa_K1486038 Nterm sfGFP N terminus moiety of split superfolder GFP
BBa_K1486039 Cterm sfGFP C terminus moiety of split superfolder GFP
BBa_K1486040 Nterm sfGFP + CpxR N terminus moiety of split sfGFP attached to CpxR
BBa_K1486041 Cterm sfGFP + CpxR C terminus moiety of split sfGFP attached to CpxR
BBa_K1486042 LZip Monomer of leucine zipper TF
BBa_K1486043 LZip + split rLuc Two Leucine Zipper monomers, each attached to a different split rLuc moiety Control for split rLuc assays


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