Team:DTU-Denmark

From 2014.igem.org

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         <td rowspan="1" scope="col" width="33%"> <h2>Problem</h2> </td>
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         <td rowspan="1" scope="col" width="33%"> <img src=https://static.igem.org/mediawiki/2014/thumb/1/18/DTU-Denmark-E_popi_solutionbig.png/316px-DTU-Denmark-E_popi_solutionbig.png> </td>
         <td rowspan="1" scope="col" width="33%"> <img src=https://static.igem.org/mediawiki/2014/thumb/1/18/DTU-Denmark-E_popi_solutionbig.png/316px-DTU-Denmark-E_popi_solutionbig.png> </td>
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        <td rowspan="1" scope="col" width="33%"> How can we quantify gene expression? </td>
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        <td rowspan="1" scope="col" width="33%"> The spinach protocol gives a flourescence dependent on expression. </td>
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        <td rowspan="1" scope="col" width="33%"> Shit worked yo. </td>
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Revision as of 11:27, 4 October 2014

Technical University of Denmark

Welcome


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Problem

Solution

Result
How can we quantify gene expression? The spinach protocol gives a flourescence dependent on expression. Shit worked yo.




Our Project


Synthetic biology is the future approach for creating biological systems with novel functions. Doing this requires a large toolbox of genetic parts, having precisely described functions.

At the DTU iGEM team 2014, we have found that characterization of one of the most central tools is missing - genetic promoters are largely uncharacterised. Because of their importance in all synthetic biology systems, it is vital that promoter strength can be characterized in detail.

During our project we seek to develop a convenient, fast and reliable method for measuring absolute promoter activities using the Spinach technology. This method relies on fluorescently tagging RNA molecules called “Spinach”. By means of the Spinach concept, we will measure the concentration of the RNA product from different promoters and use this to calculate the transcription rate of the promoters. Using standard references in our measurements will even allow us to calculate transcription rates in units of Polymerases Per Second (PoPS), which is regarded as the optimal promoter activity measure.

Thank You to Our Sponsors




Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
+45 45 25 25 25
dtuigem14@gmail.com .

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