Team:ITESM-CEM/Parts

From 2014.igem.org

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<h2>Cholesterol Oxidase</h2>
<h2>Cholesterol Oxidase</h2>
<p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.     
<p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.     
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<h2>Oxoacyl Reductase</h2>
<h2>Oxoacyl Reductase</h2>
<p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.
<p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.
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<h2>7-dehydratase</h2>
<h2>7-dehydratase</h2>
<p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.
<p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.
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</p> <br>
<h2>Neomycin Resistance</h2>
<h2>Neomycin Resistance</h2>
<p style="text-align: justify; text-justify: inter-word;"> This selective marker was gotten from a plasmid for mammalian expression, its length is of 855 nucleotides,and it was isolated from pcDNA3.1(+)/myc-His A.
<p style="text-align: justify; text-justify: inter-word;"> This selective marker was gotten from a plasmid for mammalian expression, its length is of 855 nucleotides,and it was isolated from pcDNA3.1(+)/myc-His A.
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<h2>BGHPA</h2>
<h2>BGHPA</h2>
<p style="text-align: justify; text-justify: inter-word;"> Stop coding for eucaryotic cells
<p style="text-align: justify; text-justify: inter-word;"> Stop coding for eucaryotic cells
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<h2>PCMV</h2>
<h2>PCMV</h2>

Revision as of 15:31, 2 October 2014

TEC-CEM | Parts

ITESM-CEM | Enzy7-K me

Parts 3256
 

Our Parts

The main goal of our project was to establish the construct which will help us to degrade 7-ketocholesterol consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related with atherosclerosis.



Cholesterol Oxidase

This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.


Oxoacyl Reductase

This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.


7-dehydratase

This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin.


Neomycin Resistance

This selective marker was gotten from a plasmid for mammalian expression, its length is of 855 nucleotides,and it was isolated from pcDNA3.1(+)/myc-His A.


BGHPA

Stop coding for eucaryotic cells


PCMV

Promoter from Human Cytomegalovirus, this promoter works only on eucaryotic cells.





 

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