Team:BNU-China/home.html
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<li> <a href="https://static.igem.org/mediawiki/2014/b/bf/Bnu_0135.jpg" rel="prettyPhoto" title="This pictures show the chemotaxis of different E.coli in different acids."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/6/68/Bnu_c5.jpg" alt="" /> </a> </li> | <li> <a href="https://static.igem.org/mediawiki/2014/b/bf/Bnu_0135.jpg" rel="prettyPhoto" title="This pictures show the chemotaxis of different E.coli in different acids."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/6/68/Bnu_c5.jpg" alt="" /> </a> </li> | ||
- | <li> <a href="https://static.igem.org/mediawiki/2014/f/f8/Bnu_cb6.jpg" rel="prettyPhoto" title=" | + | <li> <a href="https://static.igem.org/mediawiki/2014/f/f8/Bnu_cb6.jpg" rel="prettyPhoto" title="Capillary assay:The capillary tubes were incubated in the bacteria solution ."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/f/f3/Bnu_c6.jpg" alt="" /> </a> </li> |
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Revision as of 13:38, 2 October 2014
What We Do
Nitrogen fixing ability of rhizobium is significant for the growth of grass family. The stronger of its ability, the less chemical fertilizer will be used. Molybdenum is a key integral part of nitrogen fixing enzyme in rhizobium and Mo is also important for the growth of plants themselves. However, Mo is heavy mental. The abuse of it will cause environment pollution. Hence, spot application of Mo is of great importance. A plasmid was transfer into E.coli Promethus to make it express fusion protein, one side of which anchors on the out membrane and the other side of which,ModA,catches molybdate. With the help of organic acid tendency system, our Promethus will head for the roots. A suicide system depending on time is also design for the reason of bio-safety. E.coli Promethus will deliver Mo directly to the root of plants. This will also open a new chapter of biological fertilizer.
Wiki Design
Art design, pictures, figures and the latest experiment results were put on the wiki in time. Wiki is an important platform to show our works.
ModA & INPN
ModA we used in our project is a kind of protein that can bind the molybdate and INPN can display ModA on E.coli's surface.
Chemotaxis
We Used McfR plasmid to give the E.coli the capacity of Chemotaxis, so that those E.coli with molybdate can swim to the root of peanut.
Modeling
We have made the model of peanut root and E.coli in environment to show how our thought be realized out of lab.
Latest Works
The Story of E.coli Prometheus
BNU-China