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Team:TU Delft-Leiden/Project/Life science/EET/cloning
From 2014.igem.org
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<h6>Important note </h6> | <h6>Important note </h6> | ||
It is important to notice that the actual assembly used the 5 fragments PCRed from the original Ajo-Franklin plasmid containing the mtrCAB genes. In the figure it might seem that this happened in two steps, but that is due to the Software used to check the assembly "in silico" (SnapGene), which was limited to an assembly of four fragments. | It is important to notice that the actual assembly used the 5 fragments PCRed from the original Ajo-Franklin plasmid containing the mtrCAB genes. In the figure it might seem that this happened in two steps, but that is due to the Software used to check the assembly "in silico" (SnapGene), which was limited to an assembly of four fragments. | ||
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<img src="https://static.igem.org/mediawiki/2014/2/29/TUDelft_2014_PSB3k3_mtrCAB_History.png" width="100%" height="100%"> | <img src="https://static.igem.org/mediawiki/2014/2/29/TUDelft_2014_PSB3k3_mtrCAB_History.png" width="100%" height="100%"> | ||
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<h3> ccmAH </h3> | <h3> ccmAH </h3> | ||
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{{:Team:TU_Delft-Leiden/Templates/End}} | {{:Team:TU_Delft-Leiden/Templates/End}} | ||
Revision as of 21:28, 30 September 2014
Extracellular Electron Transport Module - Cloning
Final constructs
The final BioBricks constructed for this module were:mtrCAB
mtrCAB - His tag
ccmAH
mtrCAB
Important note
It is important to notice that the actual assembly used the 5 fragments PCRed from the original Ajo-Franklin plasmid containing the mtrCAB genes. In the figure it might seem that this happened in two steps, but that is due to the Software used to check the assembly "in silico" (SnapGene), which was limited to an assembly of four fragments.
mtrCAB - His tag
ccmAH
