Team:Hong Kong HKUST/wetlab

From 2014.igem.org

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<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
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<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:
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  can be found here!
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(1) (Detecting Module) ComD & ComE, (2) (Detecting Module) P<sub><i>comCDE</i></sub>, (3) (Regulation Module) ComX & ComW, and
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(4) (Regulation Module) combox. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively
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and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.
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The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process,
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some experiments could not be completed due to time constrains and they are outlined in Future Work page.
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<h4> Mutagenesis</h4>
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<p> Protocol description/picture here. </p>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<h4> Western Blot </h4>
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<h4> LB agar and LB plates </h4>
 
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<h4> Ligation </h4>
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<p> Protocol description/picture here. </p>
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<h4> Ethanol Precipitation</h4>
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<p> Protocol description/picture here. </p>
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<h4> Pbad protocols </h4>
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<p>Protocol description/picture here. </p>
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<h4> Gibson Assembly </h4>
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<h4> Polymerase Chain Reaction (PCR)</h4>
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Revision as of 14:50, 30 September 2014

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Wetlab

The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) (Detecting Module) ComD & ComE, (2) (Detecting Module) PcomCDE, (3) (Regulation Module) ComX & ComW, and (4) (Regulation Module) combox. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows. The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in Future Work page.

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