Team:Wageningen UR/notebook/protocols
From 2014.igem.org
(Difference between revisions)
(Created page with "{{CSS/Main}} {{:Team:Wageningen_UR/templates/header}} <html> </html> {{:Team:Wageningen_UR/templates/menu}} <html> <h3>Coming soon.</h3><br/><br/> <img src="http://2014.igem.o...") |
|||
Line 1: | Line 1: | ||
- | { | + | <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01//EN" "http://www.w3.org/TR/html4/strict.dtd"> |
+ | <html lang="en"> | ||
+ | <head> | ||
+ | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> | ||
+ | <title>Wageningen UR iGEM 2014 </title> | ||
+ | <!--<link rel="stylesheet" href="https://2014.igem.org/Team:Wageningen_UR/css?action=raw&ctype=text/css" type="text/css" />--> | ||
+ | <link rel="stylesheet" href="css/wur.css" /> | ||
+ | <!--[if (lte IE 6)|(gte IE 8)]> | ||
+ | <style type="text/css"> | ||
+ | #wrapper {height:100%;display:table;} | ||
+ | </style> | ||
+ | <![endif]--> | ||
+ | |||
+ | <!--[if IE 9]> | ||
+ | <style type="text/css"> | ||
+ | #innerwrap {margin-left: 200px;} | ||
+ | </style> | ||
+ | <![endif]--> | ||
- | + | ||
- | < | + | <script type="text/javascript" src="https://2014.igem.org/Team:Wageningen_UR/js/wur.js?action=raw&ctype=text/javascript"></script> |
+ | <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.0/jquery.min.js"></script> | ||
+ | <script type="text/javascript" src="https://2014.igem.org/Team:Wageningen_UR/js/jquery.scrollto.js?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2014.igem.org/Team:Wageningen_UR/js/colorbox.js?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2014.igem.org/Team:Wageningen_UR/js/timeliner.min.js?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="http://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script> | ||
+ | </head> | ||
+ | <body> | ||
+ | <div id="wrapper"> | ||
+ | <!--<div id="header"> | ||
+ | |||
+ | </div> --> | ||
+ | <div id="innerwrap"> | ||
+ | |||
+ | <div id="left"> | ||
+ | <p> </p> | ||
+ | <div id="inner-left"> | ||
+ | <p> </p><!-- To make sure the div doesn't float away into the sunset~ --> | ||
+ | <div class="sidebar-container"> | ||
+ | <p> </p> | ||
+ | <div id="nav-anchor"></div> | ||
+ | <sidenav> | ||
+ | <ul> | ||
+ | <!-------------------- First template section end ------------------------------------> | ||
- | </ | + | <div class="menu-head"> |
+ | <h4><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#header">Protocols</a></h4> | ||
+ | </div> | ||
- | + | <li class="menu-item"> | |
- | < | + | <a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#original">Original</a> |
- | < | + | </li> |
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <li class="menu-item"> |
- | </ | + | <a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#self-made">Self-Made</a> |
+ | </li> | ||
+ | <!-------------------- Second template section start ------------------------------------> | ||
+ | </ul> | ||
+ | </sidenav> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="center"> | ||
+ | <div id="header"> | ||
+ | </div> | ||
+ | |||
+ | <div class="navmenubkg"> | ||
+ | <div id="nav_wrapper"> | ||
+ | <div id="pblogo" class="sidelogos"> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR" title="Home Page"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/e/e5/Wageningen_UR_home.png"/> | ||
+ | </a> | ||
+ | </div> | ||
+ | |||
+ | <div id="ca-menu"> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR/overview"> | ||
+ | <span id="ca-icon1"></span> | ||
+ | <div class="ca-content">Overview</div> | ||
+ | </a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/background">Background</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/solution">Our solution</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/results">Results</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/achievements">Achievements</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/parts">Parts</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR/project"> | ||
+ | <span id="ca-icon2"></span> | ||
+ | <div class="ca-content">Project</div> | ||
+ | </a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing">Fungal Sensing</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_inhibition">Fungal Inhibition</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing">Wetlab</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing">Greenhouse</a></li> | ||
+ | </ul> | ||
+ | |||
+ | </li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/kill-switch">Kill-Switch</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/gene_transfer">Gene Transfer</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">Model</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">Promoter Design</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">System Cost</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">System Stability</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/interlab">Interlab Study</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR/outreach"> | ||
+ | <span id="ca-icon3"></span> | ||
+ | <div class="ca-content">Outreach</div> | ||
+ | </a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/policy">Policy</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/collaboration">Collaboration</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/synenergene">Synenergene</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/interviews">Interviews</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/meetup">National Meetup</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/media">Media</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR/team"> | ||
+ | <span id="ca-icon4"></span> | ||
+ | <div class="ca-content">Team</div> | ||
+ | </a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/members">Members</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/members#students">Students</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/members#">Supervisors</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/gallery">Gallery</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/attributions">Attributions</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/acknowledgements">Acknowledgements</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR/safety"> | ||
+ | <span id="ca-icon5"></span> | ||
+ | <div class="ca-content">Safety</div> | ||
+ | </a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/safety/laboratory">Laboratory</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/safety/environment">Environment</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2014.igem.org/Team:Wageningen_UR/notebook"> | ||
+ | <span id="ca-icon6"></span> | ||
+ | <div class="ca-content">Notebook</div> | ||
+ | </a> | ||
+ | <ul> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/journal">Journal</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols">Protocols</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/links">Interesting Links</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | <div id="rightbarlogo"> | ||
+ | <a href="http://www.igem.org"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/0/02/Wageningen_UR_igem_bw.png" height="45px"> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div id="rightlogo" class="sidelogos"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/b/b8/Wageningen_UR_turning_cogs_once.gif"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="center_content"> | ||
+ | <!-------------------- Second template section end ------------------------------------> | ||
- | {{: | + | <!-- Tour page example --> |
+ | |||
+ | |||
+ | <h1>Protocols</h1> | ||
+ | <br/> | ||
+ | <p>During iGEM, we used several existing protocols. We also developed a number of new protocols. On this page, both can be found</p> | ||
+ | <br><br> | ||
+ | <section id="original"> | ||
+ | <h2>Original Protocols</h2> | ||
+ | <br> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/d/d0/Wageningen_UR_protocols_Mediabuffers.pdf">Media & Buffers</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/8/8f/Wageningen_UR_protocols_Transformations.pdf">Transformations</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/1/12/Wageningen_UR_protocols_Usingkits.pdf">Using Kits</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/e/eb/Wageningen_UR_protocols_Pcr.pdf">PCR</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/1/15/Wageningen_UR_protocols_Restrictionligation.pdf">Restrictions & Ligations</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/b/b5/Wageningen_UR_protocols_Site_Directed_Mutagenesis.pdf">Mutagenesis</a></h3> | ||
+ | </section> | ||
+ | <br><br> | ||
+ | </section id="self-made"> | ||
+ | <h2>Self-Made Protocols</h2> | ||
+ | <br> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/8/87/Wageningen_UR_protocols_ammoniumanalysis.pdf">Ammonium Analysis</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/0/07/Wageningen_UR_protocols_DAPGprotocols.pdf">DAPG Protocols</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_FAgrowth.pdf">Fusarium Inhibition</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/4/4b/Wageningen_UR_protocols_MgLgrowth.pdf">MgL Growth Protocol</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/7/7a/Wageningen_UR_protocols_Pyoverdinegrowth.pdf">Pyoverdine Growth Protocol</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/2/23/Wageningen_UR_protocols_Fusariuminhibition.pdf">Fusaric Acid inducible promoter growth experiment protocol</a></h3> | ||
+ | <h3><a href="https://static.igem.org/mediawiki/2014/8/8e/Wageningen_UR_protocols_Promotercharacterisation.pdf">Promoter Characterisation Protocol</a></h3> | ||
+ | </section> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- End of table example--> | ||
+ | </p> | ||
+ | <!-------------------- Third template section end ------------------------------------> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="right"> | ||
+ | <div id="inner-right"> | ||
+ | <a href="#header" ><img id="mascot" src="https://static.igem.org/mediawiki/2014/b/bd/Wageningen_UR_mascot.png" alt="" width="110px"/></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div><!--end innerwrap--> | ||
+ | <div id="center-faux"></div> | ||
+ | </div><!--end wrapper--> | ||
+ | |||
+ | <div id="footer"> | ||
+ | <p>iGEM Wageningen UR 2014. Follow us on <a class="soft_link" href="http://twitter.com/igemwageningen">Twitter</a> and <a class="soft_link" href="http://www.facebook.com/iGEMWageningen">Facebook</a>! | ||
+ | <br /> | ||
+ | For more information about iGEM, check out their <a class="soft_link" href="https://igem.org">website</a> and <a class="soft_link" href="http://twitter.com/igem">Twitter</a>!</p> | ||
+ | </div> | ||
+ | |||
+ | <script> | ||
+ | $(document).ready(function(){ | ||
+ | /** | ||
+ | * This part handles the highlighting functionality. | ||
+ | * We use the scroll functionality again, some array creation and | ||
+ | * manipulation, class adding and class removing, and conditional testing | ||
+ | */ | ||
+ | var aChildren = $("sidenav li").children(); // find the a children of the list items | ||
+ | var aArray = []; // create the empty aArray | ||
+ | for (var i=0; i < aChildren.length; i++) { | ||
+ | var aChild = aChildren[i]; | ||
+ | var ahref = $(aChild).attr('href'); | ||
+ | aArray.push(ahref); | ||
+ | } // this for loop fills the aArray with attribute href values | ||
+ | |||
+ | $(window).scroll(function(){ | ||
+ | var windowPos = $(window).scrollTop(); // get the offset of the window from the top of page | ||
+ | var windowHeight = $(window).height(); // get the height of the window | ||
+ | var docHeight = $(document).height(); | ||
+ | |||
+ | for (var i=0; i < aArray.length; i++) { | ||
+ | var theID = aArray[i]; | ||
+ | var divPos = $(theID).offset().top; // get the offset of the div from the top of page | ||
+ | var divHeight = $(theID).height(); // get the height of the div in question | ||
+ | if (windowPos >= divPos){ | ||
+ | if (windowPos < (divPos + divHeight)) { | ||
+ | $("a[href='" + theID + "']").addClass("sidenav-active"); | ||
+ | } | ||
+ | else { | ||
+ | $("a[href='" + theID + "']").removeClass("sidenav-active"); | ||
+ | } | ||
+ | } else { | ||
+ | $("a[href='" + theID + "']").removeClass("sidenav-active"); | ||
+ | } | ||
+ | } | ||
+ | |||
+ | if(windowPos + windowHeight == docHeight) { | ||
+ | if (!$("sidenav li:last-child a").hasClass("sidenav-active")) { | ||
+ | var sidenavActiveCurrent = $(".sidenav-active").attr("href"); | ||
+ | $("a[href='" + sidenavActiveCurrent + "']").removeClass("sidenav-active"); | ||
+ | $("sidenav li:last-child a").addClass("sidenav-active"); | ||
+ | } | ||
+ | } | ||
+ | }); | ||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | <script> | ||
+ | $(document).ready(function() { | ||
+ | $.timeliner({ | ||
+ | startOpen:['#02w1EX', '#02w2EX'] <!--Initial opened journal--> | ||
+ | }); | ||
+ | $.timeliner({ | ||
+ | timelineContainer: '#timelineContainer_2' | ||
+ | }); | ||
+ | // Colorbox Modal | ||
+ | $(".CBmodal").colorbox({inline:true, initialWidth:100, maxWidth:682, initialHeight:100, transition:"elastic",speed:750}); | ||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | <script> | ||
+ | $(document).ready(function() { | ||
+ | $(".onoffswitch-checkbox").click(function() { | ||
+ | $(".overview >div img.top").toggleClass("transparent"); | ||
+ | }); | ||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | </body> | ||
+ | </html> |
Revision as of 09:09, 14 October 2014
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01//EN" "http://www.w3.org/TR/html4/strict.dtd">
Protocols
During iGEM, we used several existing protocols. We also developed a number of new protocols. On this page, both can be found