Team:EPF Lausanne/Applications
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The bacterial biosensor allowing the demonstration of our idea was a stress responsive two component regulatory system: the CpxA-R pathway. Our team successfully showed that spatiotemporal dynamics of the biosensor was possible upon fusion of split IFP1.4 fragment to the relay protein of the pathway, CpxR. | The bacterial biosensor allowing the demonstration of our idea was a stress responsive two component regulatory system: the CpxA-R pathway. Our team successfully showed that spatiotemporal dynamics of the biosensor was possible upon fusion of split IFP1.4 fragment to the relay protein of the pathway, CpxR. | ||
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<h2>Relationship between genes and their corresponding activating signals</h2> | <h2>Relationship between genes and their corresponding activating signals</h2> | ||
<p>Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.</p> | <p>Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.</p> | ||
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Revision as of 13:35, 28 September 2014
Applications
Basic Sciences Related
Protein Complementation techniques & Biosensors
Think quick ! That's the message that the EPF Lausanne iGEM team wants to convey. The BioPad project is centered around the use of Protein Complementation Techniques to enable in vivo spatiotemporal analysis of biological signals by bacterial biosensors.
But seriously, what does that mean ? Protein complementation is a technique consisting of the association of reporter protein fragments to components of a same macromolecular complex. Upon reconstitution of the macromolecular structure (active state), the unfolded fused reporter fragments are physically brought together to allow their proper folding. This allows the reconstitution of their chemical properties. The most widely used protein complementation techniques are related to fluorescence (GFP, YFP, RFP), bioluminescence (firefly, renilla luciferases), and cAMP production (Adenylyl cyclase). Most scientists use this technique to validate protein interaction in the context of signal cascades or other pathways.
The EPF Lausanne iGEM team distinguishes itself from this train of thought, as our team implemented a novel split fluorescent reporter to assess the spatiotemporal dynamics of bacterial biosensors. The fluorescent protein studied by our iGEM team is the split IFP1.4. The IFP1.4 (engineered Infrared Fluorescent Protein) is a fluorescent protein developed early in 2014 by the Michnick Lab (REFERENCE). The split IFP1.4 is the first of its kind as it is both fluorescent and reversible (most fluorescent proteins are irreversible). The reversibility is possible as its chromophore - biliverdin - is an organic molecule to which the protein binds. Moreover, the IFP1.4 has advantage of having very low background noise as fluorescence in the far-red spectrum is limited.
The bacterial biosensor allowing the demonstration of our idea was a stress responsive two component regulatory system: the CpxA-R pathway. Our team successfully showed that spatiotemporal dynamics of the biosensor was possible upon fusion of split IFP1.4 fragment to the relay protein of the pathway, CpxR.
Microfluidics as an interface for the in vivo study of Biosensors
Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.
Relationship between genes and their corresponding activating signals
Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.
Applied Sciences Related
Cheap, fast, efficient, and accurate antibiotic screening system
Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.
Antibiotic Complement
Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.
Tumor progression evalutation
Lorem ipsum dolor sit amet, an mei erant possit, nec cibo eligendi perfecto eu. Ad mea odio omnesque voluptua, summo suscipit ne est. Graeco commodo accusamus eam ut, et has esse scripta persius, eum unum virtute in. Sed ea elit errem, vocibus argumentum mel cu. Tota vivendum nam id, congue consulatu adolescens at pri. Te quo quod meis suscipiantur. Principes omittantur vis no. Omnis elitr eu pro, et qui debitis philosophia. Utinam oporteat sensibus ei usu. Ad legere adipisci vim. Vel harum primis iudicabit an, ridens debitis eu vis. Minimum consequuntur mei ut, id sea oratio volutpat, qui an fuisset deleniti adipisci. Vix ad sale fastidii, no usu graeco adolescens. No sit mnesarchum concludaturque, ne duo facete offendit. Eos ut mundi nostrum elaboraret, ut ludus delicatissimi qui. Duo te latine lucilius oportere, liber lobortis recteque sea ea.