Revision as of 22:30, 27 September 2014

WELCOME TO iGEM 2014!

2014 Project Description: Waterloo’s 2014 iGEM project aims to remove antibiotic resistance in MRSA populations by using CRISPR and RNA interference systems that target mecA expression. The gene mecA in MRSA is responsible for it’s resistance against beta-lactam antibiotics. CRISPRi and RNAi systems can be incorporated onto a Staphylococcus conjugative plasmid, which would be able to replicate and transfer within a MRSA population, effectively disabling the antibiotic resistant phenotype in the population over time. A beta-lactam antibiotic can then be administered to kill the newly antibiotic-sensitive bacteria. To execute this idea, the project was divided into three milestones. The first milestone aims to model the ability of CRISPRi and RNAi to inhibit gene expression in the level one organism, Staphylococcus epidermidis. The second milestone aims to develop an effective conjugative plasmid in Staphylococcus to ensure a proper delivery mechanism for CRISPRi and/or RNAi. Measuring the effectiveness of the conjugative plasmid will allow us to apply our current mathematical models to determine the optimal time periods to apply the antibiotics to the population after the addition of the donor cells. The third milestone would be to evaluate and improve this system for MRSA populations.
Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!

Click here to edit this page!

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Requirements

Please be sure to keep these links, your audience will want to find your:

There are a few wiki requirements teams must follow:

All pages, images and files must be hosted on the 2014.igem.org server.
All pages must be created under the team’s name space.
As part of your documentation, keep the links from the menu to the left.
Do not use flash in wiki code.
The iGEM logo should be placed on the upper part of every page and should link to 2014.igem.org.

Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

Tips

We are currently working on providing teams with some easy to use design templates.
In the meantime you can also view other team wikis for inspiration! Here are some very good examples

For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists.

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

State your accomplishments! Tell people what you have achieved from the start.
Be clear about what you are doing and what you plan to do.
You have a global audience! Consider the different backgrounds that your users come from.
Make sure information is easy to find; nothing should be more than 3 clicks away.
Avoid using very small fonts and low contrast colors; information should be easy to read.
Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2013 calendar
Have lots of fun!

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 Waterloo’s 2014 iGEM project aims to remove antibiotic resistance in MRSA populations by using CRISPR and RNA interference systems that target mecA expression. The gene mecA in MRSA is responsible for it’s resistance against beta-lactam antibiotics. CRISPRi and RNAi systems can be incorporated onto a Staphylococcus conjugative plasmid, which would be able to replicate and transfer within a MRSA population, effectively disabling the antibiotic resistant phenotype in the population over time. A beta-lactam antibiotic can then be administered to kill the newly antibiotic-sensitive bacteria.
 To execute this idea, the project was divided into three milestones. The first milestone aims to model the ability of CRISPRi and RNAi to inhibit gene expression in the level one organism, Staphylococcus epidermidis. The second milestone aims to develop an effective conjugative plasmid in Staphylococcus to ensure a proper delivery mechanism for CRISPRi and/or RNAi. Measuring the effectiveness of the conjugative plasmid will allow us to apply our current mathematical models to determine the optimal time periods to apply the antibiotics to the population after the addition of the donor cells. The third milestone would be to evaluate and improve this system for MRSA populations.