Team:WashU StLouis/Notebook/July

From 2014.igem.org

(Difference between revisions)
(Created page with "{{:Team:WashU_StLouis/CSS}} {{WashU/Layout}} <html> <style> #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: no...")
Line 16: Line 16:
<tr> <td>
<tr> <td>
<h1> <center><b> July</b> </center></h1>
<h1> <center><b> July</b> </center></h1>
-
 
+
<body>
-
<p> insert data here </p>
+
<br>
-
 
+
<table
 +
style="text-align: left; width: 80%; height: 1300px; margin-left: auto; margin-right: auto;"
 +
border="1" cellpadding="5" cellspacing="0">
 +
<tbody>
 +
<tr>
 +
<td style="vertical-align: top;"><img
 +
style="width: 300px; height: 400px;" alt="Week 5"
 +
src="https://static.igem.org/mediawiki/2014/9/9b/WashU_Week_5.jpg"><br>
 +
</td>
 +
<td style="vertical-align: top;">
 +
<div style="text-align: center;"><span style="font-weight: bold;">Week
 +
Five- June 30- July 6</span><br>
 +
</div>
 +
Team starts re-culturing the strains in more nutritious LB media.&nbsp;
 +
Prepare 50% glycerol solution for storage and autoclaved water as well
 +
as the M9 medium. Caroline continues to work on scripts and outreach
 +
videos. Growth of all strains is significantly improved. <br>
 +
Brauer Group has not had success with chromophore plasmid yet,
 +
redesigned the plasmid by piecing together different components.
 +
Started with cloning of proposed hybrid promoter and sent in for
 +
sequence verification.<br>
 +
Had our third worldly Wednesday at Gyro House- Mediterranean cuisine. </td>
 +
</tr>
 +
<tr>
 +
<td style="vertical-align: top;"><img
 +
style="width: 300px; height: 405px;" alt="Week 6"
 +
src="https://static.igem.org/mediawiki/2014/8/88/WashU_Week_6.jpg"><br>
 +
</td>
 +
<td style="vertical-align: top;">
 +
<div style="text-align: center;"><span style="font-weight: bold;">Week
 +
Six- July 7-13</span><br>
 +
</div>
 +
After series of culturing testing, the best media, optimal nitrogen
 +
source, optimal carbon source, optimal temperature for culturing are
 +
all determined. C-source: Glucose. N-source: Glutamate. Culturing
 +
Media: LB. Testing Media: M9. Temperature: 30 Celsius (Anaerobic).
 +
&nbsp;<br>
 +
Brauer group continued work on chromophore plasmid. Started design of
 +
experimentation without the chromophore as a sanity check. Ptet
 +
promoter seemed to be giving our plasmids issues as it did not
 +
fluoresce as expected. Contacted Tabor group for possibly shipping the
 +
plasmid to WashU.<br>
 +
Had a mid summer progress report meeting with the professors and
 +
explained to them our work’s progress and difficulties we faced.<br>
 +
Had our fourth Worldly Wednesday at Asian Kitchen- Korean cuisine.</td>
 +
</tr>
 +
<tr>
 +
<td style="vertical-align: top;"><img
 +
style="width: 300px; height: 400px;" alt="Week 7 picture"
 +
src="https://static.igem.org/mediawiki/2014/a/af/WashU_Week_7.jpg"><br>
 +
</td>
 +
<td style="vertical-align: top;">
 +
<div style="text-align: center;"><span style="font-weight: bold;">Week
 +
Seven- July 14-20</span><br>
 +
</div>
 +
Before the formal examination (Acetylene Reduction Assay) for the
 +
nitrogenase activity, the team performed plasmid extraction and gel
 +
running to confirm the existence of plasmid inside the strains. <br>
 +
Brauer Group worked on cloning for degradation tags and other side
 +
projects. Continued conversations with shipping plasmid to WashU and
 +
also found a promising Biobrick part and ordered from the registry. <br>
 +
Ben started working on a math model of our system with Brandon.<br>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td style="vertical-align: top;"><img
 +
style="width: 300px; height: 405px;" alt="Week 8 picture"
 +
src="https://static.igem.org/mediawiki/2014/2/2c/WashU_Week_8.jpg"><br>
 +
</td>
 +
<td style="vertical-align: top;">
 +
<div style="text-align: center;"><span style="font-weight: bold;">Week
 +
Eight- July 21-27</span><br>
 +
</div>
 +
Rebstock group ran the Acetylene Reduction Assay with GC machine for
 +
all strains including negative control. Specific procedure can be found
 +
in protocol. <br>
 +
Brauer group received the biobrick part and started cloning a new
 +
plasmid for the chromophore part, and sent in for sequencing.<br>
 +
Ben continued work on math model with Brandon by sending necessary
 +
information to him and writing ODEs.<br>
 +
The team had our fifth worldly Wednesday at De Palm Tree- Jamaican
 +
cuisine… spicy!!<br>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td style="vertical-align: top;"><img
 +
style="width: 300px; height: 493px;" alt="Week 9 pic"
 +
src="https://static.igem.org/mediawiki/2014/e/e3/WashU_Week9.jpg"><br>
 +
</td>
 +
<td style="vertical-align: top;">
 +
<div style="text-align: center;"><span style="font-weight: bold;">Week
 +
Nine- July 28- August 3</span><br>
 +
</div>
 +
Continued Acetylene Reduction Assays for all strains and controls. <br>
 +
Brauer group continued troubleshooting of chromophore plasmid. And sent
 +
off degradation tag plasmids for sequencing as well. Designed an
 +
experiment for light induction by co-transforming chromophore plasmids
 +
and light regulation plasmids. Make necessary plates for experiment and
 +
started growing cultures for experimentation.</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
 +
</body>
</td>
</td>
</tr>
</tr>

Revision as of 21:42, 26 September 2014



Special thanks to our 2014 Sponsors:

If you want to sponsor us, please contact us below!

© Washington University in St. Louis iGEM 2014 | Contact Us | Follow Us on Social Media

Retrieved from "http://2014.igem.org/Team:WashU_StLouis/Notebook/July"