Team:WashU StLouis/Notebook/June

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<h1> <center><b> June </b> </center></h1>
<h1> <center><b> June </b> </center></h1>
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<p> insert data here </p>
 
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<body>
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<div style="text-align: center;">WashU iGEM Group Log<br>
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</div>
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<br>
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<table
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style="text-align: left; width: 80%; height: 1300px; margin-left: auto; margin-right: auto;"
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border="1" cellpadding="5" cellspacing="0">
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<tbody>
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<tr>
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<td
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style="vertical-align: top; height: 100px; text-align: center;"><img
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style="width: 300px; height: 300px;" alt="Monsanto visit"
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src="https://static.igem.org/mediawiki/2014/2/2c/WashU_Monsanto_visit.JPG"><br>
</td>
</td>
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<td style="vertical-align: top;">
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<div style="text-align: center;"><span style="font-weight: bold;">Week
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One- June 2-8</span><br>
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</div>
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At the beginning of the first week, we gathered as a group to finalize
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our summer timeline. We had to give a presentation to faculty advisors
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on our summer readings. <br>
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We created team nitroGENIUS, and prepared a timeline for the summer as
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well.<br>
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Later, the team&nbsp; traveled to Monstanto, one of out major sponsors,
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Headquarters and gave a presentation to a panel of nitrogen fixation
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experts on our summer project, and got a tour of the facilities.
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Afterwards we started lab safety training and split into our respective
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labs for the summer.<br>
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The Brauer team also started design of cloning for light regulation
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mechanism and ordered primers.</td>
</tr>
</tr>
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<tr>
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<td style="vertical-align: top;"><img
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style="width: 300px; height: 400px;" alt="Week 2 pic"
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src="https://static.igem.org/mediawiki/2014/3/3f/WashU_Week_2.jpg"><br>
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</td>
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<td style="vertical-align: top;">
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<div style="text-align: center;"><span style="font-weight: bold;">Week
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Two- June 9-15</span><br>
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</div>
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Continued from Week One, the team was trained to make LB media and
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completed all of the basic lab procedure training in their respective
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labs. During the second week, the team also completed the LB plate
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preparation. Rebstock Team members were trained for transformation of
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designed plasmid to JM109, BL21(DE3), Top10, and DH5α was also done.
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Started filling preliminary Safety forms. &nbsp;<br>
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Continued work on creating negative and positive controls for light
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regulation, in addition to the light regulation plasmid and the
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necessary precursor plasmid.<br>
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At the end of the week we had our first meeting with Brandon and Rajib
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from Penn State to see how they can help our project from a
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computational perspective; they looked at possibly implementing a
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minimal nif cluster once we have the nitrogen fixation working.</td>
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</tr>
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<tr>
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<td style="vertical-align: top;"><img
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style="width: 300px; height: 400px;" alt="Colony PCR"
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src="https://static.igem.org/mediawiki/2014/7/7b/WashU_Week_3.jpg"><br>
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</td>
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<td style="vertical-align: top;">
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<div style="text-align: center;"><span style="font-weight: bold;">Week
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Three- June 16-22</span><br>
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</div>
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Electroporation and transformation was done to all strains—plasmids
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are
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now in all selected strains. Rebstock Team started culturing
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experimental plates and tubes of JM109, BL21(DE3), Top10, and DH5α
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with
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plasmids.&nbsp; Positive and Negative control are designed. Team also
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initiated to contact Dennis Dean and two Chinese research groups about
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their plasmid that can be utilized as positive control for WashU iGEM
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team. <br>
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Brauer group sent positive and negative controls for sequencing and
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learned how to colony PCR. Also redesigned plasmid and primers for
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light regulation.<br>
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We had another meeting with Brandon and Rajib; this time giving them an
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update on our wet lab work progress.<br>
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Had our first Worldly Wednesday at the Taj Mahal.</td>
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</tr>
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<tr>
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<td style="vertical-align: top;"><img
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style="width: 300px; height: 300px;" alt="week 4"
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src="https://static.igem.org/mediawiki/2014/c/c4/WashU_Week4.JPG"><br>
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</td>
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<td style="vertical-align: top;">
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<div style="text-align: center;"><span style="font-weight: bold;">Week
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Four- June 23-29</span><br>
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</div>
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As part of outreach program, Caroline starts to work on the scripts of
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video series introducing WashU iGEM project. Video filming is also
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initiated and in progress well.&nbsp; Rebstock Team continued culturing
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the
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strains growing in plates in the hot room into new tubes as preparation
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for inoculation. Media used is minimal M9 media. Growth of the wild
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type strains is very limited and the engineered strains achieved low
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growth as well.<br>
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Brauer group continued cloning for negative control and finished
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cloning of light regulation plasmid and sent in for sequence
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verification. Keep troubleshooting the chromophore plasmid. <br>
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We had our second Worldly Wednesday at Queen of Sheba- Ethiopian food.
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Also watched a world cup match as a group.<br>
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Brandon updated us on his progress of looking at protein domains in
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different nif clusters. Considered starting a model of our system. Our
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group explained to him our progress and sent him information on our
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protocols</td>
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</tr>
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</tbody>
</table>
</table>
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<br>
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<br>
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<br>
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</body>
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</td>

Revision as of 21:39, 26 September 2014