Team:Carnegie Mellon/Superoxide

From 2014.igem.org

(Difference between revisions)
(Description of project and list of methods added)
Line 103: Line 103:
<h1><center>Superoxide Generator Improvement</center> </h1>
<h1><center>Superoxide Generator Improvement</center> </h1>
-
<p> <center>Description...</center></p>
+
<p> <center>In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods<a href="https://2013.igem.org/Team:Carnegie_Mellon/Project/Abstract">(Source: Carnegie Mellon iGEM 2013)</a></center>.</p>
 +
 
 +
<h4>Methods Used</h4>
 +
<ol>
 +
  <li>Optimization of codons in KillerRed by eliminating rare codon usage.
 +
  <li>Optimization of codons in Supernova, the monomeric version of KillerRed, by eliminating rare codon usage.
 +
  <li>Photobleaching analysis of KillerRed, Codon Optimized KillerRed, and Codon Optimized Supernova.
 +
</ol>
<hr>
<hr>
<hr>
<hr>

Revision as of 16:48, 29 September 2014

Carousel Template · Bootstrap

Superoxide Generator Improvement

In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods(Source: Carnegie Mellon iGEM 2013)
.

Methods Used

  1. Optimization of codons in KillerRed by eliminating rare codon usage.
  2. Optimization of codons in Supernova, the monomeric version of KillerRed, by eliminating rare codon usage.
  3. Photobleaching analysis of KillerRed, Codon Optimized KillerRed, and Codon Optimized Supernova.

Week by Week Notebook Entries

Retrieved from "http://2014.igem.org/Team:Carnegie_Mellon/Superoxide"