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Team:Uppsala/Project Notebook
From 2014.igem.org
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],["<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>16 restreaks of transformation from 140715</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>red</td><td>O/N from frozen stock</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>O/N from frozen stock</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>cheZ</td><td>O/N from frozen stock</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-USP45-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23102-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23114-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>Restreak</td><td>S.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>Restreak</td><td>S.H</td></tr>", | ],["<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>16 restreaks of transformation from 140715</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>red</td><td>O/N from frozen stock</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>O/N from frozen stock</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>cheZ</td><td>O/N from frozen stock</td><td>D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-USP45-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23102-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23114-B0034-CFY</td><td>screening</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>Restreak</td><td>S.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>Restreak</td><td>S.H</td></tr>", | ||
],["<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-USP45-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23102-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>OVernight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23114-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>Screening</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>Screening</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-pgsA-CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-pgsA</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP1-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP11-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP29-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td></td><td>red</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>Overnight</td><td>T.R</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>Overnight</td><td>T.R</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP1-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP11-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP29-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td></td><td>red</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>PCR screening 34.4.1-34.4.11 & 34.8-34.22</td><td>J.R. E.S. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>O/N cultures for 34.8-34.23</td><td>J.R. E.S. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>SOB</td><td>A.G/T.R</td></tr>", | ],["<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-USP45-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23102-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>OVernight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23114-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>Screening</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>Screening</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-pgsA-CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-pgsA</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP1-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP11-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP29-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td></td><td>red</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>Overnight</td><td>T.R</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>Overnight</td><td>T.R</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP1-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP11-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP29-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td></td><td>red</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>PCR screening 34.4.1-34.4.11 & 34.8-34.22</td><td>J.R. E.S. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>O/N cultures for 34.8-34.23</td><td>J.R. E.S. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>SOB</td><td>A.G/T.R</td></tr>", | ||
| - | ],[22],[ | + | ],["<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-USP45-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pelB-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-USP45-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23102-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23106-B0034-CFY</td><td>Overnight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23113-B0034-CFY</td><td>OVernight</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23114-B0034-CFY</td><td>digest+ligation+transformation+screning</td><td>A.W+M. H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>Screening</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_WT-B0034-BFP</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>Screening</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>yenbox_J23113-B0034-BFP</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR</td><td>O/N</td><td></td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-pgsA-CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>CFY</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-pgsA</td><td>Digestion</td><td>T.R/T.HE</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP1-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP11-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP29-B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td></td><td>red</td><td>Ligation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>Overnight</td><td>T.R</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>Overnight</td><td>T.R</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP1-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP11-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>CP29-B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pjp059 (C and E resistance)</td><td>CP11-B0034-CFY</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td></td><td>red</td><td>Transformation</td><td>T.R/V.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>PCR screening 34.4.1-34.4.11 & 34.8-34.22</td><td>J.R. E.S. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>O/N cultures for 34.8-34.23</td><td>J.R. E.S. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>SOB</td><td>A.G/T.R</td></tr>", |
| + | ],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR-yenbox_WT-B0034-BFP</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR-yenbox_J23113-B0034-BFP</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR-yenbox_WT-B0034-BFP</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR-yenbox_J23113-B0034-BFP</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113-B0034</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114-B0034</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23100-B0034</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0032-GFP</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23115</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB3K3</td><td>J23101-B0032-GFP</td><td>Restreak</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5a</td><td>pSB1K3</td><td>B0034-cheZ</td><td>digestion (34.4.2, 34.4.7, 34.11, 34.12, 34.17)</td><td>E.S., M.S. (?)</td></tr>","<tr><td></td><td>BBa_J61002</td><td>J23100-red</td><td>digestion</td><td>E.S., M.S. (?)</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23100-B0034-cheZ</td><td>ligation (34.4.2, 34.4.7, 34.11, 34.12, 34.17)</td><td>E.S. M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23100-B0034-cheZ</td><td>transformation (34.4.2, 34.4.7, 34.11, 34.12, 34.17)</td><td>E.S. M.S.</td></tr>","<tr><td></td><td></td><td></td><td>Plates</td><td>M.F/G.C</td></tr>","<tr><td></td><td></td><td></td><td>LB agar</td><td>M.F/G.C/M.B</td></tr>","<tr><td></td><td></td><td></td><td>LB</td><td>M.F/G.C/M.B</td></tr>", | ||
| + | ],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23100-B0034-cheZ</td><td>transformation (34.12 + promoter)</td><td>E.S</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-cheZ</td><td>transformation with digested & intact 34.12 as p/c and n/c</td><td>E.S</td></tr>","<tr><td>DH5a</td><td>pSB1K3</td><td>B0034-cheZ</td><td>ligation</td><td>J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1K3</td><td>B0034-cheZ</td><td>transformation</td><td>J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>BBa_J61002</td><td>J23113</td><td>digestion</td><td>J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>BBa_J61002</td><td>J23114</td><td>digestion</td><td>J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23113-B0034-CheZ</td><td>ligation</td><td>E.S., J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23114-B0034-CheZ</td><td>ligation</td><td>E.S., J.R., M.S.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR-yenbox_WT-B0034-BFP</td><td>Screening</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101-B0034-YenR-yenbox_J23113-B0034-BFP</td><td>Screening</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR-yenbox_WT-B0034-BFP</td><td>Screening</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23110-B0034-YenR-yenbox_J23113-B0034-BFP</td><td>Screening</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113-B0034</td><td>Screening/ON</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114-B0034</td><td>Screening/ON</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23100-B0034</td><td>Screening/ON</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0032-GFP</td><td>Screening/ON</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23115</td><td>Screening/ON</td><td>M.F/S.H/M.B/G.C</td></tr>","<tr><td>DH5-alfa</td><td>pSB3K3</td><td>J23101-B0032-GFP</td><td>Screening/ON</td><td>M.F/S.H/M.B/G.C</td></tr>", | ||
| + | ],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113-B0034</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114-B0034</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23100-B0034</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0032-GFP</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23115</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB3K3</td><td>J23101-B0032-GFP</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23100-B0034-cheZ</td><td>transformation</td><td>E.S., J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23113-B0034-CheZ</td><td>transformation</td><td>E.S., J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23114-B0034-CheZ</td><td>transformation</td><td>E.S., J.R., M.S.</td></tr>", | ||
| + | ],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113-B0034</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114-B0034</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23100-B0034</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0032-GFP</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23115</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5-alfa</td><td>pSB3K3</td><td>J23101-B0032-GFP</td><td>Plasmid prep</td><td>GC</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23100-B0034-cheZ</td><td>transformation</td><td>E.S., J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23113-B0034-CheZ</td><td>transformation</td><td>E.S., J.R., M.S.</td></tr>","<tr><td>DH5a</td><td>pSB1C3</td><td>J23114-B0034-CheZ</td><td>transformation</td><td>E.S., J.R., M.S.</td></tr>", | ||
| + | ],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5], | ||
]; | ]; | ||
</script> | </script> | ||
Revision as of 19:29, 24 September 2014
Stephanie Herman
Teresa Reinli
Joakim Hellner
Alexander Virtanen
Jennifer Rosenius
Marcus Hong
Miranda Stiernborg
Tim Hagelby Edström
Viktor Blomkvist
Megha Biradar
Niklas Handin
Jonas Mattisson
Arina Gromov
Nils Anlind
Eric Sandström
Gunta Celma
Oliver Possnert
Martin Friberg
Kira Karlsson
Christoffer Andersson
Laura Pacoste
Andries Willem Boers
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