Team:Uppsala/Project Notebook
From 2014.igem.org
(Difference between revisions)
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],["<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_wt</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_J23113</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_wt-B0034-BFP</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_J23113-B0034-BFP</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutPCR (SynBio protocol, ext. time = 90 sec), second try</td><td>E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>Plates</td><td>C.A M.H M.F A.G</td></tr>","<tr><td></td><td></td><td></td><td>LB agar</td><td>M.H</td></tr>", | ],["<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_wt</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_J23113</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_wt-B0034-BFP</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_J23113-B0034-BFP</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutPCR (SynBio protocol, ext. time = 90 sec), second try</td><td>E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>Plates</td><td>C.A M.H M.F A.G</td></tr>","<tr><td></td><td></td><td></td><td>LB agar</td><td>M.H</td></tr>", | ||
],["<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutPCR (SynBio protocol, ext. time = 90 sec, with 0-8% DMSO)</td><td>E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>frozen stocks</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>frozen stocks</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>frozen stocks</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>competent cells</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>competent cells</td><td>M.S. D.B.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test from O/N culture on T swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test from O/N culture on T swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test from O/N culture on T swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>CFY</td><td>Restreak</td><td>A.G</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>LuxR</td><td>Restreak</td><td>L.P</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>GenR</td><td>Restreak</td><td>L.P</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>RBS_1AC7</td><td>Restreak</td><td>J.M</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>RBS-LacZ-fragment</td><td>Restreak</td><td>L.P</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>B0034_USP45_CFY</td><td>Restreak</td><td>A.G</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>B0034_PelB_CFY</td><td>Restreak</td><td>A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>lux pL-B0034-luxR-lux pR - BFP</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>J23100-B0034-LuxI</td><td>ligation and transformation</td><td>MB</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-LuxI</td><td>ligation and transformation</td><td>MB</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>J23110-B0034-LuxI</td><td>ligation and transformation</td><td>MB</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-USP45-CFY (bad suffix)</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>", | ],["<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutPCR (SynBio protocol, ext. time = 90 sec, with 0-8% DMSO)</td><td>E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>frozen stocks</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>frozen stocks</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>frozen stocks</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>competent cells</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>competent cells</td><td>M.S. D.B.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test from O/N culture on T swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test from O/N culture on T swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test from O/N culture on T swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>CFY</td><td>Restreak</td><td>A.G</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>LuxR</td><td>Restreak</td><td>L.P</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>GenR</td><td>Restreak</td><td>L.P</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>RBS_1AC7</td><td>Restreak</td><td>J.M</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>RBS-LacZ-fragment</td><td>Restreak</td><td>L.P</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>B0034_USP45_CFY</td><td>Restreak</td><td>A.G</td></tr>","<tr><td>d5-alpha</td><td>pSB1C3</td><td>B0034_PelB_CFY</td><td>Restreak</td><td>A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>lux pL-B0034-luxR-lux pR - BFP</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>J23100-B0034-LuxI</td><td>ligation and transformation</td><td>MB</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>J23101-B0034-LuxI</td><td>ligation and transformation</td><td>MB</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>J23110-B0034-LuxI</td><td>ligation and transformation</td><td>MB</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-USP45-CFY (bad suffix)</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>O/N</td><td>M.H A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>Screening</td><td>M.H L.P J.M A.G</td></tr>", | ||
- | ],[ | + | ],["<tr><td>DH5-alpha</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pSB3T5</td><td>CFY</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pJP059 (C and E resistance)</td><td>red</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>J23102</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>J23106</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>J23113</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>J23114</td><td>Plasmidprep</td><td>A.G</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>B0034-pgsA-CFY</td><td>Ligation</td><td>L.P T.HE</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>B0034-pgsA-GFP</td><td>Ligation</td><td>L.P T.HE</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>S.H./G.C.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-YenR</td><td>screening</td><td>S.H./G.C.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>RBS-LacZ alpha fragment</td><td>screening</td><td>s.H./G.C.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ alpha fragment</td><td>O/N</td><td>S.H./G.C.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ</td><td>screening</td><td>s.H./G.C.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>YenBox_wt</td><td>Ligation</td><td>M.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>YenBox_J23113</td><td>Ligation</td><td>M.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>YenBox_wt-B0034-BFP</td><td>Ligation</td><td>M.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>YenBox_23113-B0034-BFP</td><td>Ligation</td><td>M.B/M.F</td></tr>","<tr><td>DH5-alpha</td><td>pSB1K3</td><td>J23110-B0034-LuxI</td><td>screening</td><td>s.H./G.C.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1K3</td><td>J23110-B0034-LuxI</td><td>O/N</td><td>S.H./G.C.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>GFP fusion brick</td><td>Digest</td><td></td></tr>","<tr><td>DH5-alpha</td><td>pSB1A3</td><td>CFY</td><td>Digest</td><td></td></tr>","<tr><td>RP1616</td><td>pSB1C3</td><td>red</td><td>transformation</td><td>E.Q. K.K.</td></tr>","<tr><td>UU2685</td><td>pSB1C3</td><td>red</td><td>transformation</td><td>E.Q. K.K.</td></tr>","<tr><td>DH5alfa</td><td>pSB3T5</td><td>red</td><td>restreak practice</td><td>D.B. K.K. E.Q.</td></tr>", |
+ | ],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5], | ||
]; | ]; | ||
</script> | </script> |
Revision as of 18:58, 24 September 2014
Stephanie Herman
Teresa Reinli
Joakim Hellner
Alexander Virtanen
Jennifer Rosenius
Marcus Hong
Miranda Stiernborg
Tim Hagelby Edström
Viktor Blomkvist
Megha Biradar
Niklas Handin
Jonas Mattisson
Arina Gromov
Nils Anlind
Eric Sandström
Gunta Celma
Oliver Possnert
Martin Friberg
Kira Karlsson
Christoffer Andersson
Laura Pacoste
Andries Willem Boers
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