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Team:Uppsala/Project Notebook
From 2014.igem.org
(Difference between revisions)
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],["<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>ON</td><td>J.M</td></tr>", | ],["<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>ON</td><td>J.M</td></tr>", | ||
],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-BFP</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pL-B0034-luxR-lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-YenR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Plasmid prep</td><td>J.M</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Screening</td><td>J.M</td></tr>","<tr><td>dH5-alpha</td><td>pSB1K3</td><td>B0034-CheZ</td><td>2% agarose gel to assess restriction success</td><td>D.B. E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>", | ],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-BFP</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pL-B0034-luxR-lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-YenR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Plasmid prep</td><td>J.M</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Screening</td><td>J.M</td></tr>","<tr><td>dH5-alpha</td><td>pSB1K3</td><td>B0034-CheZ</td><td>2% agarose gel to assess restriction success</td><td>D.B. E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>", | ||
| - | ],[ | + | ],["<tr><td></td><td></td><td></td><td>Tryptone swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutagenic PCR (SynBio protocol, ext. time = 60 sec)</td><td>E.Q.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutagenic PCR (SynBio protocol, ext. time = 90 sec)</td><td>E.Q.</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_wt + pSB1C3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_J23113 + pSB1C3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_wt + B0034-BFP + pSB1A3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_J23113 + B0034-BFP + pSB1A3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>lux pL-B0034-luxR-lux pR - BFP</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-YenR</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-YenR</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ alpha fragment</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>GFP fusion brick</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A res</td><td>J23112-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23113-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23109</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23114-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23105-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23106-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23100-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA (bad suffix)</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA (bad suffix)</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-USP45-CFY (bad suffix)</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>CFY</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pelB-CFY (Bad Suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pelB-CFY (Bad Suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-GFP (bad suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-GFP (bad suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-CFY (bad suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-CFY (bad suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA</td><td>pSB1C3</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA</td><td>pSB1C3</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td></td><td></td><td></td><td></td><td></td></tr>", |
| + | ],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5], | ||
]; | ]; | ||
</script> | </script> | ||
Revision as of 18:53, 24 September 2014
Stephanie Herman
Teresa Reinli
Joakim Hellner
Alexander Virtanen
Jennifer Rosenius
Marcus Hong
Miranda Stiernborg
Tim Hagelby Edström
Viktor Blomkvist
Megha Biradar
Niklas Handin
Jonas Mattisson
Arina Gromov
Nils Anlind
Eric Sandström
Gunta Celma
Oliver Possnert
Martin Friberg
Kira Karlsson
Christoffer Andersson
Laura Pacoste
Andries Willem Boers
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