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| | <td colspan="3" rowspan="3" align="left" valign="top"><ul> | | <td colspan="3" rowspan="3" align="left" valign="top"><ul> |
| - | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Interlab</a></sub> | + | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Safety</a></sub> |
| - | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Existing GFP device</a></sub> | + | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab"></a></sub> |
| | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 1</a></sub> | | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 1</a></sub> |
| | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 2</a></sub> | | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 2</a></sub> |
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| | <!--INICIO CONTENIDO --> | | <!--INICIO CONTENIDO --> |
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| | <div style="background-color: #f3f3e2;"> | | <div style="background-color: #f3f3e2;"> |
| - | <table width="100%" border="0" id="ContenidoSecciones"> | + | <table width="200%" border="0" id="ContenidoSecciones"> |
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| - | <h2>Overview</h2> | + | <h2>Laboratory Biosafety </h2> |
| - | <p>During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study. | + | |
| | + | <p style="text-align: justify; text-justify: inter-word;"> Our laboratory is classified as Laboratory of Risk 1. According to iGEM safety page and COFEPRIS which is the agency in Mexico that is encharged of regulating a variety of health related topics, a laboratory of risk level 1 is the one that can hold microorganisms that can not cause a disease in humans. </p> |
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| - | The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.
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| - | Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.
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| - | The aim of this study is to report the relative efficiency of the following genetic devices:<br><br>
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| - | 1) Promoter BBa_K823005 in low-copy plasmid psB3K3<br>
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| - | 2) Promoter BBa_K823005 in high-copy plasmig psB1C3<br>
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| - | 3) Promoter BBa_K823012 in high-copy plasmid psB1C3<br><br>
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| - | In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.<br><br>
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| - | GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.
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