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Team:UESTC-China/Protocol
From 2014.igem.org
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<table border="1" id="BiosensorsTable" cellspacing="0";> | <table border="1" id="BiosensorsTable" cellspacing="0";> | ||
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| - | <th class="top"> | + | <th class="top">Component</th> |
| - | <th class="top"> | + | <th class="top">20ul Reaction</th> |
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| - | <td class="mid"> | + | <td class="mid">1.33X Master Mix</td> |
| - | + | <td class="mid"> 15ul</td> | |
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| - | <td class="mid"> | + | <td class="mid">Insert DNA </td> |
| - | + | <td class="mid"> the moles of insert DNA to vector DNA is 5：1</td> | |
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| - | <td class="mid"> | + | <td class="mid">>Vector DNA</td> |
| - | <td class="mid"> | + | <td class="mid"> 50ng</td> |
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| - | <td class="mid"> | + | <td class="mid">ddH2O</td> |
| - | <td class="mid"> | + | <td class="mid"> To 20ul</td> |
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| - | <td class="mid"> | + | <td class="mid">Incubate at 50℃ for 1 hour</td> |
| - | + | <td class="mid"> </td> | |
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</table> | </table> | ||
</div> | </div> | ||
Revision as of 04:12, 24 September 2014
DNA ligation with T4 DNA Ligase
| Component | 20ul Reaction |
|---|---|
| 1.33X Master Mix | 15ul |
| Insert DNA | the moles of insert DNA to vector DNA is 5：1 |
| >Vector DNA | 50ng |
| ddH2O | To 20ul |
| Incubate at 50℃ for 1 hour |
DNA ligation with T4 DNA Ligase
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
