Template:Kyoto/Notebook/DMS

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   <div id="kyoto-notebook">
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 +
<div>
 +
 +
<a name="0731" class="kyoto-jump"></a>
 +
<h3>7/31</h3>
 +
 +
<h4>Restriction Enzyme Digestion</h4>
 +
<span class="kyoto-author">Nakamura and Matsumoto</span>
 +
<table>
 +
<tr>
 +
<th colspan=2>Sample/(&micro;L)</th>
 +
<th>EcoR1/(&micro;L)</th>
 +
<th>Xba1/(&micro;L)</th>
 +
<th>Spe1/(&micro;L)</th>
 +
<th>Pst1/(&micro;L)</th>
 +
<th colspan=2>Buffer/(&micro;L)</th>
 +
<th>BSA/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
</tr>
 +
<tr>
 +
<td>3/24 PCR(dddD) cloning product</td>
 +
<td>3.2</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>3</td>
 +
<td>-</td>
 +
<td>21.8</td>
 +
<td>30</td>
 +
</tr>
 +
<tr>
 +
<td>7/18 RBS30@</td>
 +
<td>18.5</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>3</td>
 +
<td>-</td>
 +
<td>6.5</td>
 +
<td>30</td>
 +
</tr>
 +
<tr>
 +
<td>7/18 RBS30 control@</td>
 +
<td>6.2</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>2.8</td>
 +
<td>10</td>
 +
</tr>
 +
</table>
 +
</div>
 +
 +
<div>
 +
 +
<a name="0801" class="kyoto-jump"></a>
 +
<h3>8/1</h3>
 +
 +
<h4>Gel Extraction</h4>
 +
<span class="kyoto-author">Murata and Yasuda</span>
 +
<table>
 +
<tr>
 +
<th>Lane</th>
 +
<th>Sample</th>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>1kbp ladder</td>
 +
<td>2</td>
 +
<td>3/24 PCR(dddD) cloning product E,S</td>
 +
<td>3</td>
 +
<td>3/24 PCR(dddD) cloning product E,S</td>
 +
<td>4</td>
 +
<td>Blank</td>
 +
<td>5</td>
 +
<td>7/18 RBS30 E,S@</td>
 +
<td>6</td>
 +
<td>7/18 RBS30 E,S@</td>
 +
<td>7</td>
 +
<td>Blank</td>
 +
<td>8</td>
 +
<td>1kbp ladder</td>
 +
<td>9</td>
 +
<td>3/24 PCR(dddD) cloning product control</td>
 +
<td>10</td>
 +
<td>7/18 RBS30 control@</td>
 +
</tr>
 +
</table>
 +
<table>
 +
<tr>
 +
<th>Sample</th>
 +
<th>Concentration/(&micro;g/mL)</th>
 +
<th>260/280</th>
 +
<th>260/230</th>
 +
</tr>
 +
<tr>
 +
<td>PSB1C3</td>
 +
<td>13.3</td>
 +
<td>1.90</td>
 +
<td>-</td>
 +
</tr>
 +
<tr>
 +
<td>dddD</td>
 +
<td>10.8</td>
 +
<td>1.64</td>
 +
<td>0.15</td>
 +
</tr>
 +
</table>
 +
 +
<h4>Ligation</h4>
 +
<span class="kyoto-author">Yasuda</span>
 +
<table>
 +
<tr>
 +
<th colspan=2>Vector/(&micro;L)</th>
 +
<th colspan=2>Insert/(&micro;L)</th>
 +
<th colspan=2>Buffer/(&micro;L)</th>
 +
<th colspan=2>Ligase/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</tr>
 +
<tr>
 +
<td>8/1 PSB1C3 gel extraction product 2047bp</td>
 +
<td>1.5</td>
 +
<td>8/1 dddD gel extraction product 2545bp</td>
 +
<td>6.9</td>
 +
<td>10xT4Ligase</td>
 +
<td>2</td>
 +
<td>T4Ligase</td>
 +
<td>1</td>
 +
<td>8.6</td>
 +
<td>20</td>
 +
</tr>
 +
</table>
 +
 +
<h4>Transformation</h4>
 +
<span class="kyoto-author">Murata</span>
 +
<table>
 +
<tr>
 +
<th colspan=2>Sample/(&micro;L)</th>
 +
<th colspan=2>CompetentCells/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
<th>Medium</th>
 +
</tr>
 +
<tr>
 +
<td>PSB1C3-dddD</td>
 +
<td>3</td>
 +
<td>コンピ名</td>
 +
<td>30</td>
 +
<td>33</td>
 +
<td>SOC</td>
 +
</tr>
 +
</table>
 +
 +
</div>
 +
 +
<div>
 +
 +
<a name="0802" class="kyoto-jump"></a>
 +
<h3>8/2</h3>
 +
 +
<h4>PCR Cloning</h4>
 +
<span class="kyoto-author">Murata</span>
 +
<table>
 +
<tr>
 +
<th>@</th>
 +
<th>2x KAPA HiFi Hotstart Ready Mix/(&micro;L)</th>
 +
<th>F Pre-dddD cloning 62/(&micro;L)</th>
 +
<th>Rv Spe1-dddD cloning 68/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
</tr>
 +
<tr>
 +
<td>@</td>
 +
<td>12.5</td>
 +
<td>0.75</td>
 +
<td>0.75</td>
 +
<td>11</td>
 +
<td>25</td>
 +
</tr>
 +
</table>
 +
<table>
 +
<tr>
 +
<th>Predenature</th>
 +
<th>Denature</th>
 +
<th>Annealing</th>
 +
<th>Extension</th>
 +
</tr>
 +
<tr>
 +
<td>95@</td>
 +
<td>98@</td>
 +
<td>65@</td>
 +
<td>72@</td>
 +
</tr>
 +
<tr>
 +
<td>5min</td>
 +
<td>20sec</td>
 +
<td>15sec</td>
 +
<td>50sec</td>
 +
</tr>
 +
</table>
 +
<p>Denature-Annealing-Extension 25cycle</p>
 +
<table>
 +
<tr>
 +
<th>Number</th>
 +
<th>Sample</th>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>pSB1C3-dddD(1)</td>
 +
</tr>
 +
<tr>
 +
<td>2</td>
 +
<td>pSB1C3-dddD(2)</td>
 +
</tr>
 +
<tr>
 +
<td>3</td>
 +
<td>pSB1C3-dddD(3)</td>
 +
</tr>
 +
</table>
 +
 +
<h4>DNA Purification</h4>
 +
<span class="kyoto-author">Murata</span>
 +
<p>@</p>
 +
 +
<h4>Colony PCR</h4>
 +
<span class="kyoto-author">Honda,Nakamura and Matsumoto</span>
 +
<p>Master Mix</p>
 +
<table>
 +
<tr>
 +
<th>Quick Tag/(&micro;L)</th>
 +
<th>VF2/(&micro;L)</th>
 +
<th>VR/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
</tr>
 +
<tr>
 +
<td>15</td>
 +
<td>0.6</td>
 +
<td>0.6</td>
 +
<td>13.8</td>
 +
<td>30</td>
 +
</tr>
 +
</table>
 +
<table>
 +
<tr>
 +
<th>Predenature</th>
 +
<th>Denature</th>
 +
<th>Annealing</th>
 +
<th>Extension</th>
 +
</tr>
 +
<tr>
 +
<td>94@</td>
 +
<td>94@</td>
 +
<td>55@</td>
 +
<td>68@</td>
 +
</tr>
 +
<tr>
 +
<td>2min</td>
 +
<td>30sec</td>
 +
<td>30sec</td>
 +
<td>24sec</td>
 +
</tr>
 +
</table>
 +
<p>Denature-Annealing-Extension 30cycle</p>
 +
<p>☆</p>
 +
 +
<h4>Concentration Measurement</h4>
 +
<span class="kyoto-author">Nakamura and Matsumoto</span>
 +
<table>
 +
<tr>
 +
<th>Sample</th>
 +
<th>Concentration/(&micro;g/mL)</th>
 +
<th>260/280</th>
 +
<th>260/230</th>
 +
</tr>
 +
<tr>
 +
<td>@</td>
 +
<td>245</td>
 +
<td>1.59</td>
 +
<td>2.17</td>
 +
</tr>
 +
</table>
 +
 +
<h4>Restriction Enzyme Digestion</h4>
 +
<span class="kyoto-author">名前</span>
 +
<table>
 +
<tr>
 +
<th colspan=2>Sample/(&micro;L)</th>
 +
<th>EcoR1/(&micro;L)</th>
 +
<th>Xba1/(&micro;L)</th>
 +
<th>Spe1/(&micro;L)</th>
 +
<th>Pst1/(&micro;L)</th>
 +
<th colspan=2>Buffer/(&micro;L)</th>
 +
<th>BSA/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
</tr>
 +
<tr>
 +
<td>8/2 dddD cloning product@</td>
 +
<td>8.2</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>3</td>
 +
<td>-</td>
 +
<td>16.8</td>
 +
<td>30</td>
 +
</tr>
 +
<tr>
 +
<td>8/2 dddD cloning control@</td>
 +
<td>0.41</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>8.6</td>
 +
<td>10</td>
 +
</tr>
 +
<tr>
 +
<td>7/18 RBS30@</td>
 +
<td>12.7</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>3</td>
 +
<td>-</td>
 +
<td>12.3</td>
 +
<td>30</td>
 +
</tr>
 +
<tr>
 +
<td>7/18 RBS30 control@</td>
 +
<td>0.04</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>H</td>
 +
<td>1</td>
 +
<td>-</td>
 +
<td>8.4</td>
 +
<td>10</td>
 +
</tr>
 +
</table>
 +
 +
</div>
 +
 +
<div>
 +
 +
<a name="0803" class="kyoto-jump"></a>
 +
<h3>8/3</h3>
 +
 +
<h4>@</h4>
 +
<span class="kyoto-author">Murata</span>
 +
<table>
 +
<tr>
 +
<th>Lane</th>
 +
<th>Sample</th>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>pSB1C3-dddD(1)</td>
 +
<td>2</td>
 +
<td>pSB1C3-dddD(2)</td>
 +
<td>3</td>
 +
<td>pSB1C3-dddD(3)</td>
 +
<td>4</td>
 +
<td>1kb ladder (3&micro;L)</td>
 +
<td>5</td>
 +
<td>1kb ladder (1&micro;L)</td>
 +
</tr>
 +
</table>
 +
<h4>@</h4>
 +
<span class="kyoto-author">Tatsui</span>
 +
<table>
 +
<tr>
 +
<th>Lane</th>
 +
<th>Sample</th>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>1kb ladder</td>
 +
<td>2</td>
 +
<td>Blank</td>
 +
<td>3</td>
 +
<td>7/18 RBA30@(pSB1C3) 2070bp</td>
 +
<td>4</td>
 +
<td>Blank</td>
 +
<td>5</td>
 +
<td>7/18 RBA30@(pSB1C3)</td>
 +
<td>6</td>
 +
<td>Blank</td>
 +
<td>7</td>
 +
<td>8/2 cloning dddD 2544bp</td>
 +
<td>8</td>
 +
<td>Blank</td>
 +
<td>9</td>
 +
<td>8/2 cloning dddD</td>
 +
</tr>
 +
</table>
 +
<table>
 +
<tr>
 +
<th>Sample</th>
 +
<th>Concentration/(&micro;g/mL)</th>
 +
<th>260/280</th>
 +
<th>260/230</th>
 +
</tr>
 +
<tr>
 +
<td>7/18 RBS30(pSB1C3)@</td>
 +
<td>@</td>
 +
<td>1.37</td>
 +
<td>0.42</td>
 +
</tr>
 +
<tr>
 +
<td>8/2 cloning dddD</td>
 +
<td>35</td>
 +
<td>1.54</td>
 +
<td>0.85</td>
 +
</tr>
 +
</table>
 +
 +
<h4>Ligation</h4>
 +
<span class="kyoto-author">@</span>
 +
<table>
 +
<tr>
 +
<th colspan=2>Vector/(&micro;L)</th>
 +
<th colspan=2>Insert/(&micro;L)</th>
 +
<th colspan=2>Buffer/(&micro;L)</th>
 +
<th colspan=2>Ligase/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</tr>
 +
<tr>
 +
<td>@</td>
 +
<td>7</td>
 +
<td>@</td>
 +
<td>7</td>
 +
<td>2xBuffer</td>
 +
<td>15</td>
 +
<td>T4Ligase</td>
 +
<td>1</td>
 +
<td>@</td>
 +
<td>@</td>
 +
</tr>
 +
<tr>
 +
<td>@</td>
 +
<td>7</td>
 +
<td>@</td>
 +
<td>14</td>
 +
<td>2xBuffer</td>
 +
<td>22</td>
 +
<td>T4Ligase</td>
 +
<td>1</td>
 +
<td>@</td>
 +
<td>@</td>
 +
</tr>
 +
<tr>
 +
<td>@</td>
 +
<td>7</td>
 +
<td>@</td>
 +
<td>@</td>
 +
<td>2xBuffer</td>
 +
<td>15</td>
 +
<td>T4Ligase</td>
 +
<td>1</td>
 +
<td>7</td>
 +
<td>@</td>
 +
</tr>
 +
</table>
 +
<h4>Transformation</h4>
 +
<span class="kyoto-author">@</span>
 +
<table>
 +
<tr>
 +
<th colspan=2>Sample/(&micro;L)</th>
 +
<th colspan=2>CompetentCells/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
<th>Medium</th>
 +
</tr>
 +
<tr>
 +
<td>@</td>
 +
<td>@</td>
 +
<td>@</td>
 +
<td>@</td>
 +
<td>@</td>
 +
<td>@</td>
 +
</tr>
 +
</table>
 +
</div>
 +
 +
<div>
 +
 +
<a name="0804" class="kyoto-jump"></a>
 +
<h3>8/4</h3>
 +
 +
<h4>Colony PCR</h4>
 +
<span class="kyoto-author">Honda</span>
 +
<p>Master Mix</p>
 +
<table>
 +
<tr>
 +
<th>Quick Tag/(&micro;L)</th>
 +
<th>VF2/(&micro;L)</th>
 +
<th>VR/(&micro;L)</th>
 +
<th>MilliQ/(&micro;L)</th>
 +
<th>Total/(&micro;L)</th>
 +
</tr>
 +
<tr>
 +
<td>40</td>
 +
<td>1.6</td>
 +
<td>1.6</td>
 +
<td>36.8</td>
 +
<td>80</td>
 +
</tr>
 +
</table>
 +
<table>
 +
<tr>
 +
<th>Predenature</th>
 +
<th>Denature</th>
 +
<th>Annealing</th>
 +
<th>Extension</th>
 +
</tr>
 +
<tr>
 +
<td>94@</td>
 +
<td>94@</td>
 +
<td>55@</td>
 +
<td>68@</td>
 +
</tr>
 +
<tr>
 +
<td>2min</td>
 +
<td>30sec</td>
 +
<td>30sec</td>
 +
<td>24sec</td>
 +
</tr>
 +
</table>
 +
<p>Denature-Annealing-Extension 30cycle</p>
 +
<table>
 +
<tr>
 +
<th>1</th>
 +
<th>dddD+pSB1C3(1)-1</th>
 +
</tr>
 +
<tr>
 +
<td>2</td>
 +
<td>dddD+pSB1C3(1)-2</td>
 +
</tr>
 +
<tr>
 +
<td>3</td>
 +
<td>dddD+pSB1C3(2)-1</td>
 +
</tr>
 +
<tr>
 +
<td>4</td>
 +
<td>dddD+pSB1C3(2)-2</td>
 +
</tr>
 +
<tr>
 +
<td>5</td>
 +
<td>dddD+pSB1C3(2)-3</td>
 +
</tr>
 +
<tr>
 +
<td>6</td>
 +
<td>dddD+pSB1C3(2)-4</td>
 +
</tr>
 +
<tr>
 +
<td>7</td>
 +
<td>pSB1C3 control-1</td>
 +
</tr>
 +
<tr>
 +
<td>8</td>
 +
<td>control</td>
 +
</tr>
 +
</table>
 +
<h4>@</h4>
 +
<span class="kyoto-author">Matsumoto</span>
 +
<table>
 +
<tr>
 +
<th>Lane</th>
 +
<th>Sample</th>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>1kbp ladder</td>
 +
<td>2</td>
 +
<td>dddD+pSB1C3(1)-1</td>
 +
<td>3</td>
 +
<td>dddD+pSB1C3(1)-2</td>
 +
<td>4</td>
 +
<td>dddD+pSB1C3(2)-1</td>
 +
<td>5</td>
 +
<td>dddD+pSB1C3(2)-2</td>
 +
<td>6</td>
 +
<td>dddD+pSB1C3(2)-3</td>
 +
<td>7</td>
 +
<td>dddD+pSB1C3(2)-4</td>
 +
<td>8</td>
 +
<td>pSB1C3 control-1</td>
 +
<td>9</td>
 +
<td>control</td>
 +
<td>10</td>
 +
<td>1kbp ladder</td>
 +
</tr>
 +
</table>
 +
</div>
 +
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Revision as of 19:14, 23 September 2014

7/31

Restriction Enzyme Digestion

Nakamura and Matsumoto
Sample/(µL) EcoR1/(µL) Xba1/(µL) Spe1/(µL) Pst1/(µL) Buffer/(µL) BSA/(µL) MilliQ/(µL) Total/(µL)
3/24 PCR(dddD) cloning product 3.2 1 - 1 - H 3 - 21.8 30
7/18 RBS30@ 18.5 1 - 1 - H 3 - 6.5 30
7/18 RBS30 control@ 6.2 - - - - H 1 - 2.8 10

8/1

Gel Extraction

Murata and Yasuda
Lane Sample
1 1kbp ladder 2 3/24 PCR(dddD) cloning product E,S 3 3/24 PCR(dddD) cloning product E,S 4 Blank 5 7/18 RBS30 E,S@ 6 7/18 RBS30 E,S@ 7 Blank 8 1kbp ladder 9 3/24 PCR(dddD) cloning product control 10 7/18 RBS30 control@
Sample Concentration/(µg/mL) 260/280 260/230
PSB1C3 13.3 1.90 -
dddD 10.8 1.64 0.15

Ligation

Yasuda
Vector/(µL) Insert/(µL) Buffer/(µL) Ligase/(µL) MilliQ/(µL) Total/(µL)
8/1 PSB1C3 gel extraction product 2047bp 1.5 8/1 dddD gel extraction product 2545bp 6.9 10xT4Ligase 2 T4Ligase 1 8.6 20

Transformation

Murata
Sample/(µL) CompetentCells/(µL) Total/(µL) Medium
PSB1C3-dddD 3 コンピ名 30 33 SOC

8/2

PCR Cloning

Murata
@ 2x KAPA HiFi Hotstart Ready Mix/(µL) F Pre-dddD cloning 62/(µL) Rv Spe1-dddD cloning 68/(µL) MilliQ/(µL) Total/(µL)
@ 12.5 0.75 0.75 11 25
Predenature Denature Annealing Extension
95@ 98@ 65@ 72@
5min 20sec 15sec 50sec

Denature-Annealing-Extension 25cycle

Number Sample
1 pSB1C3-dddD(1)
2 pSB1C3-dddD(2)
3 pSB1C3-dddD(3)

DNA Purification

Murata

@

Colony PCR

Honda,Nakamura and Matsumoto

Master Mix

Quick Tag/(µL) VF2/(µL) VR/(µL) MilliQ/(µL) Total/(µL)
15 0.6 0.6 13.8 30
Predenature Denature Annealing Extension
94@ 94@ 55@ 68@
2min 30sec 30sec 24sec

Denature-Annealing-Extension 30cycle

☆

Concentration Measurement

Nakamura and Matsumoto
Sample Concentration/(µg/mL) 260/280 260/230
@ 245 1.59 2.17

Restriction Enzyme Digestion

名前
Sample/(µL) EcoR1/(µL) Xba1/(µL) Spe1/(µL) Pst1/(µL) Buffer/(µL) BSA/(µL) MilliQ/(µL) Total/(µL)
8/2 dddD cloning product@ 8.2 1 - 1 - H 3 - 16.8 30
8/2 dddD cloning control@ 0.41 - - - - H 1 - 8.6 10
7/18 RBS30@ 12.7 1 - 1 - H 3 - 12.3 30
7/18 RBS30 control@ 0.04 - - - - H 1 - 8.4 10

8/3

@

Murata
Lane Sample
1 pSB1C3-dddD(1) 2 pSB1C3-dddD(2) 3 pSB1C3-dddD(3) 4 1kb ladder (3µL) 5 1kb ladder (1µL)

@

Tatsui
Lane Sample
1 1kb ladder 2 Blank 3 7/18 RBA30@(pSB1C3) 2070bp 4 Blank 5 7/18 RBA30@(pSB1C3) 6 Blank 7 8/2 cloning dddD 2544bp 8 Blank 9 8/2 cloning dddD
Sample Concentration/(µg/mL) 260/280 260/230
7/18 RBS30(pSB1C3)@ @ 1.37 0.42
8/2 cloning dddD 35 1.54 0.85

Ligation

@
Vector/(µL) Insert/(µL) Buffer/(µL) Ligase/(µL) MilliQ/(µL) Total/(µL)
@ 7 @ 7 2xBuffer 15 T4Ligase 1 @ @
@ 7 @ 14 2xBuffer 22 T4Ligase 1 @ @
@ 7 @ @ 2xBuffer 15 T4Ligase 1 7 @

Transformation

@
Sample/(µL) CompetentCells/(µL) Total/(µL) Medium
@ @ @ @ @ @

8/4

Colony PCR

Honda

Master Mix

Quick Tag/(µL) VF2/(µL) VR/(µL) MilliQ/(µL) Total/(µL)
40 1.6 1.6 36.8 80
Predenature Denature Annealing Extension
94@ 94@ 55@ 68@
2min 30sec 30sec 24sec

Denature-Annealing-Extension 30cycle

1 dddD+pSB1C3(1)-1
2 dddD+pSB1C3(1)-2
3 dddD+pSB1C3(2)-1
4 dddD+pSB1C3(2)-2
5 dddD+pSB1C3(2)-3
6 dddD+pSB1C3(2)-4
7 pSB1C3 control-1
8 control

@

Matsumoto
Lane Sample
1 1kbp ladder 2 dddD+pSB1C3(1)-1 3 dddD+pSB1C3(1)-2 4 dddD+pSB1C3(2)-1 5 dddD+pSB1C3(2)-2 6 dddD+pSB1C3(2)-3 7 dddD+pSB1C3(2)-4 8 pSB1C3 control-1 9 control 10 1kbp ladder

Retrieved from "http://2014.igem.org/Template:Kyoto/Notebook/DMS"