WikitemplateB project

From 2014.igem.org

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<h3>Car Pools</h3>
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<h3>One E.coli Has One Function
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<p>Imagine huge bodies of water, giant ponds and lakes and just below the surface are trillions of organisms working 24/7, eating plant life and producing gasoline. - George Church, envisioning future synthetic biofuel production </p>
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<p>Synthetic biology assumes a future for modified organisms beyond the lab. Biofuel research is currently using both natural and genetically engineered algae to generate gasoline, with the goal of one day being available for public use. The research has created a network of open-ponds for algae production. Dispersed across the southwest of the United States, companies are utilizing the environment’s abundance of sunshine - ideal algae growing conditions. As synthetic biology moves out of the lab, to the wild, to factories, to garage labs, to people’s homes what are the potential ecological effects associated with the release of a modified organism? </p>
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What is our project at 2014?
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<p>Car Pools is a series of simulations that examine the potential ecological effects associated with the public release of genetically altered algae for biofuel production. The project draws on current open-pond algae production methods to imagine a future fuel-pool infrastructure for the city of Los Angeles, a metropolis built for cars and where there are more than 43,000 swimming pools. </p>
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Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli.
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Concretely, we use two kinds of E.coli. One catches Cadmium.　the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli).
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To use these E.coli. Finally cadomiun is catched(wastewater is be clean).
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<p>The pool is typically viewed as a symbol of suburban leisure, Car Pools recasts it, like the new garage lab, into a site of homegrown fuel production. The oil wells of tomorrow may be in sunny California. The project plays out different scales of interaction, the home, the neighborhood, and the city, to explore potential ecological effects, such as pool wildlife & production management, neighborhood contamination, expanded pool networks, and modified commuting patterns. What if simulations at different scale, from micro to macro were used within synthetic biology? How could simulations be used to expose both the issues and opportunities beyond the lab?</p>
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What experiment we did?
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・How to check E.coli's chemotaxis?
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We make use of other igem team’s　assay.
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Using soft agar plate.we let E.coli siwm.
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・We made a plasmid
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Our project need to two kind of plasmid.
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we made one plasmid.And we have to make one more plasmid.
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Revision as of 10:38, 28 September 2014

Retrieved from "http://2014.igem.org/WikitemplateB_project"

One E.coli Has One Function

What is our project at 2014? Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli. Concretely, we use two kinds of E.coli. One catches Cadmium.　the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli). To use these E.coli. Finally cadomiun is catched(wastewater is be clean). What experiment we did? ・How to check E.coli's chemotaxis? We make use of other igem team’s　assay. Using soft agar plate.we let E.coli siwm. ・We made a plasmid Our project need to two kind of plasmid. we made one plasmid.And we have to make one more plasmid.