|
|
| Line 3: |
Line 3: |
| | | | |
| | <html> | | <html> |
| - | | + | <head> |
| - | <!--main content --> | + | <title>Team:Missouri Miners - 2014.igem.org</title> |
| - | <table width="70%" align="center"> | + | <link href='http://fonts.googleapis.com/css?family=Abel' rel='stylesheet' type='text/css'> |
| - | | + | <link href='http://fonts.googleapis.com/css?family=Oswald' rel='stylesheet' type='text/css'> |
| - | | + | <link rel="stylesheet" type="text/css" href="css.css" /> |
| - | <!--welcome box -->
| + | <meta name="description" content="iGEM Missouri Miners 2014: Fixing nitrogen nitrogen oxide pollutants in coal flue gases using synthetic biology" /> |
| - | <tr>
| + | <meta http-equiv="content-type" content="text/html; charset=utf-8" /> |
| - | <td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
| + | <meta name="viewport" content="width=device-width" /> |
| - | <h1 >WELCOME TO iGEM 2014! </h1>
| + | </head> |
| - | <p>Your team has been approved and you are ready to start the iGEM season! | + | <body> |
| - | <br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
| + | <div id="wiki"> |
| - | <br>
| + | <a href="/Team:Missouri_Miners"> Page</a><a href="/ Talk:Missouri_Miners"> Discussion</a><a href="/ wiki/ index. php?title=Team:Missouri_Miners &action= edit"> Source</a><a href="/ wiki/ index. php?title=Team:Missouri_Miners &action= history"> History</a><a href="https:// 2012.igem.org/ wiki/ index. php?title= Special:UserLogin&returnto=Team:Missouri_Miners"> Log in</a> |
| - | <p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Missouri_Miners&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p>
| + | </div> |
| - | </td>
| + | <div id="logo"><img src="images/mm-logo.png" alt="Missouri Miners iGEM" /></div> |
| - | </tr>
| + | <div id="menu"> |
| - | | + | <div id="igem-logo"> |
| - | <tr> <td colspan="3" height="5px"> </td></tr>
| + | <a href="https://2014.igem.org/ Main_Page">< img src=" images/igem -logo. png" alt="iGEM 2014 Home" /></a> |
| - | <!-- end welcome box -->
| + | </div> |
| - | <tr>
| + | <a href=" home. html"> Home</a><a href=" project. html">Project</a><a href=" parts. html">Parts</a>< label /><a href=" team. html"> Team</a><a href=" notebook. html">Notebook</a><a href=" safety. html">Safety</a> |
| - | | + | </div> |
| - | <!--navigation menu -->
| + | <div id="page"> |
| - | <td align="center" colspan="3">
| + | <h1>Missouri S&T iGEM</h1> |
| - | | + | <p> Coal produces a significant portion of global power, but also of pollution. Nitrogen oxides, formed and released into the atmosphere by coal burning, deplete the ozone layer and oxidize in air to form acid rain. The ozone layer absorbs the vast majority of ultraviolet radiation from the sun, shielding organisms on the surface from these destructive, cancer-causing rays. After extreme depletion, regulation and responsible practices have the ozone layer slowly recovering, but our ability to affect and harm the environment is now more apparent than ever. Similarly, human contributions to acid rain have been attributed to aquatic ecosystem destruction, soil spoiling, and structure corrosion. Nitric oxides themselves cause numerous respiratory symptoms.</p> |
| - | <table width="100%">
| + | <h3>Our Project</h3> |
| - | <tr heigth="15px"></tr>
| + | <p>We aimed to genetically modify < i> Cyanothece</ i> to allow it to remove all forms of nitrogen oxides from coal flue gases. Fixing nitrogen through < i> Cyanothece</ i> not only prevents these harmful compounds from being introduced to the atmosphere, but produces ammonia compounds as end products. Ammonia is an essential part of fertilizer, providing a path to offset costs of cleaning emissions. We standardized these nitrogen fixing genes and added them to the BioBrick Parts Registry for use by other synthetic biologists, hoping that our work is the first step to eliminating nitrogen oxide pollutants.</ p> |
| - | <tr heigth="75px">
| + | </div> |
| - | | + | </body> |
| - | | + | </html> |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners"style="color:#000000">Home </a> </td> | + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Team"style="color:#000000"> Team </a> </td> | + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
| - | <a href="https://igem.org/Team.cgi?year=2014&team_name=Missouri_Miners"style="color:#000000"> Official Team Profile </a></td> | + | |
| - | | + | |
| - | <td style="border:1px solid black" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> | + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Project"style="color:#000000"> Project</a></td>
| + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> | + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Parts"style="color:#000000"> Parts</a></td> | + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> | + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Modeling"style="color:#000000"> Modeling</a></td> | + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Notebook"style="color:#000000"> Notebook</a></td> | + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Safety"style=" color:#000000"> Safety </a></td> | + | |
| - | | + | |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
| - | <a href="https://2014.igem.org/Team:Missouri_Miners/Attributions"style="color:#000000"> Attributions </a></td> | + | |
| - | | + | |
| - | | + | |
| - | <td align ="center"> <a href="https:// 2014.igem.org/ Main_Page"> <img src="https:/ /static. igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width= "55px"></a > </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
| - | | + | |
| - | <!--end navigation menu -->
| + | |
| - | </tr>
| + | |
| - | | + | |
| - | | + | |
| - | </tr>
| + | |
| - |
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | </td>
| + | |
| - | | + | |
| - | <tr> <td colspan="3" height="15px"> </td></tr>
| + | |
| - | <tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
| + | |
| - | <tr> <td colspan="3" height="5px"> </td></tr>
| + | |
| - | | + | |
| - | | + | |
| - | <!--project description --> | + | |
| - | <tr><td colspan="3"> <h3> Project Description </h3></td></tr>
| + | |
| - | <tr> | + | |
| - | <td width="45%" valign="top">
| + | |
| - | | + | |
| - | <p> The goal of this year's project is to genetically modify Cyanothece 51142 to allow it to fix all forms of nitrogen oxide pollutants in coal flue gases. Coal is one of the primary sources of global energy and all that burning produces a lot of carbon dioxide and nitrogen oxide pollutants, among others, which contribute to global warming and acid rain formation. Fixing nitrogen through Cyanothece 51142 has another benefit besides reducing pollution; the end products are ammonia compounds which are a major component of fertilizer. We hope this will allow power companies to offset some of the costs of cleaning their emissions by selling the ammonia products to be made into fertilizer. We will be standardizing the nitrogen fixing genes and adding them to the BioBricks Parts Registry for the Jamboree next year. </p>
| + | |
| - | | + | |
| - | </td>
| + | |
| - | | + | |
| - | <!--requirements section -->
| + | |
| - | <tr><td colspan="3"> <h3> Requirements </h3></td></tr>
| + | |
| - | <tr>
| + | |
| - | <td width="45%" valign="top">
| + | |
| - | | + | |
| - | <p> Please be sure to keep these links, your audience will want to find your: </p>
| + | |
| - | | + | |
| - | <!-- Links to other team pages -->
| + | |
| - | <ul>
| + | |
| - | <li><a href="https://2014.igem.org/ Team:Missouri_Miners"> Home< /a> </li> | + | |
| - | <li><a href="https://2014.igem.org/Team:Missouri_Miners/Team">Team</a> </li>
| + | |
| - | <li><a href=" https://igem. org/Team.cgi?year=2013&team_name=Missouri_Miners"> Official Team Profile</a > </li> | + | |
| - | <li><a href=" https://2014. igem.org/Team:Missouri_Miners/Project">Project</a > </li> | + | |
| - | <li><a href=" https://2014. igem.org/Team:Missouri_Miners/Parts">Parts</a> </ li> | + | |
| - | <li><a href=" https://2014. igem.org/Team:Missouri_Miners/Modeling"> Modeling</a > </li> | + | |
| - | <li><a href=" https://2014. igem.org/Team:Missouri_Miners/Notebook">Notebook</a > </li> | + | |
| - | <li><a href=" https://2014. igem.org/Team:Missouri_Miners/Safety">Safety</a> </ li> | + | |
| - | <li><a href="https://2014.igem.org/Team:Missouri_Miners/Attributions">Attributions</a> </li> | + | |
| - | | + | |
| - | </ul> | + | |
| - | | + | |
| - | </td> | + | |
| - | | + | |
| - | <td > </td>
| + | |
| - | <td width="45%">
| + | |
| - | | + | |
| - | <p>There are a few wiki requirements teams must follow:</p> | + | |
| - | <ul>
| + | |
| - | <li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload"> 2014.igem.org server</a>. </li>
| + | |
| - | <li>All pages must be created under the team’s name space.</li>
| + | |
| - | <li>As part of your documentation, keep the links from the menu to the left. </li>
| + | |
| - | <li>Do not use flash in wiki code. </li>
| + | |
| - | <li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
| + | |
| - | </ul>
| + | |
| - | <p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
| + | |
| - | | + | |
| - | </td>
| + | |
| - | </tr>
| + | |
| - | | + | |
| - | | + | |
| - | <tr> <td colspan="3" height="15px"> </td></tr>
| + | |
| - | <tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
| + | |
| - | | + | |
| - | | + | |
| - | <!--tips -->
| + | |
| - | <tr><td colspan="3" > <h3> Tips </h3></td></tr>
| + | |
| - | | + | |
| - | <tr>
| + | |
| - | <td width="45%" valign="top">
| + | |
| - | <p>We are currently working on providing teams with some easy to use design templates. | + | |
| - | <br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p> | + | |
| - | | + | |
| - | <ul>
| + | |
| - | <li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
| + | |
| - | <li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
| + | |
| - | <li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
| + | |
| - | <li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
| + | |
| - | <li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
| + | |
| - | </ul>
| + | |
| - | | + | |
| - | <p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
| + | |
| - | </td>
| + | |
| - | | + | |
| - | <td> </td>
| + | |
| - | <td width="45%">
| + | |
| - | | + | |
| - | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
| + | |
| - | | + | |
| - | <ul>
| + | |
| - | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
| + | |
| - | <li>Be clear about what you are doing and what you plan to do.</li>
| + | |
| - | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
| + | |
| - | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
| + | |
| - | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
| + | |
| - | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
| + | |
| - | <li>Have lots of fun! </li>
| + | |
| - | </ul>
| + | |
| - | </br>
| + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
Missouri S&T iGEM
Coal produces a significant portion of global power, but also of pollution. Nitrogen oxides, formed and released into the atmosphere by coal burning, deplete the ozone layer and oxidize in air to form acid rain. The ozone layer absorbs the vast majority of ultraviolet radiation from the sun, shielding organisms on the surface from these destructive, cancer-causing rays. After extreme depletion, regulation and responsible practices have the ozone layer slowly recovering, but our ability to affect and harm the environment is now more apparent than ever. Similarly, human contributions to acid rain have been attributed to aquatic ecosystem destruction, soil spoiling, and structure corrosion. Nitric oxides themselves cause numerous respiratory symptoms.
Our Project
We aimed to genetically modify Cyanothece to allow it to remove all forms of nitrogen oxides from coal flue gases. Fixing nitrogen through Cyanothece not only prevents these harmful compounds from being introduced to the atmosphere, but produces ammonia compounds as end products. Ammonia is an essential part of fertilizer, providing a path to offset costs of cleaning emissions. We standardized these nitrogen fixing genes and added them to the BioBrick Parts Registry for use by other synthetic biologists, hoping that our work is the first step to eliminating nitrogen oxide pollutants.
"
