Team:Wageningen UR

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<p>Pathogens belonging to the Fursarium species are known to infect a wide range of crops and cause large losses in agriculture. In this project, we will concentrate on protecting bananas from Fusarium oxysporum f.sp. cubense using an engineered strain of the native soil bacterium Pseudomonas putida. At present commercially grown bananas for export are all the same species, known as Cavendish. Prior to the 1960s, though, the Gros Michel cultivar was the primary banana for export. This species was almost completely wiped out within a decade by the Panama disease that is caused by Fusarium. Recently a new strain of Fusarium has been infecting Cavendish plants and is spreading throughout the world.</p>
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<h1><b>Saving the bananas</b></h1>
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<p>Every time you go to the supermarket or to the local market to buy bananas you take for granted that all banana boxes will be full and ready for you to enjoy its amazing taste. However, a world without bananas is more plausible than we all might imagine. Banana plants are threatened by <i>Fusarium oxisporum</i>, a soil pathogen that affects plants entering through its root system causing its death. Bananas, apart from being a delicious fruit, have a great economic importance in many tropical regions. These are the reasons why the <b>iGEM Wageingen team 2014</b> has designed and created <b> BananaGuard</b>, a genetic system in <i>Pseudomonas putida</i> which is able to detect the presence of <i>Fuasarium</i> in the soil and produce a combination of antifungals that will inhibit the growth of the pathogen. If you want to read more about the system and our work during the last six months, the <b>iGEM Wageningen team 2014</b> invites you to continue surfing around our Wiki.</p>
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<p>The first and perhaps most important part is the production of fungal growth inhibitors. In order to prevent these fungal inhibitors from damaging the soil microbiome, their production will be coupled to the sensing of fusaric acid, a characteristic toxin produced by Fusarium. To help our host compete against the fungus, resistance against fusaric acid will also be incorporated.</p>
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<p>Protecting the natural biodiversity is a big part of our project. When the Fusarium threat has been detected and subsequently cleaned up, the bacteria will activate a kill-switch. In addition to this, spread of the genetically modified material will be prevented by a double dependent plasmid system in which loss or gain of one of the two modified plasmids will cause the host to kill itself.</p>
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Revision as of 17:24, 21 September 2014

Wageningen UR iGEM 2014

 

 

 

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Saving the bananas

Every time you go to the supermarket or to the local market to buy bananas you take for granted that all banana boxes will be full and ready for you to enjoy its amazing taste. However, a world without bananas is more plausible than we all might imagine. Banana plants are threatened by Fusarium oxisporum, a soil pathogen that affects plants entering through its root system causing its death. Bananas, apart from being a delicious fruit, have a great economic importance in many tropical regions. These are the reasons why the iGEM Wageingen team 2014 has designed and created BananaGuard, a genetic system in Pseudomonas putida which is able to detect the presence of Fuasarium in the soil and produce a combination of antifungals that will inhibit the growth of the pathogen. If you want to read more about the system and our work during the last six months, the iGEM Wageningen team 2014 invites you to continue surfing around our Wiki.

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