Team:Evry/Interlab Study/09-18-2014

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<i>PSB1C3+E0240+J23107</i>: <br>
<i>PSB1C3+E0240+J23107</i>: <br>
glycerol stock preparation from LB culture from the 17th September.  
glycerol stock preparation from LB culture from the 17th September.  
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<i>PSB1C3+E0240+J23110</i>: <br>
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Pre-culture does'nt grow. The TECAN measure is reported.
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<i>PSB1C3+E0240+J23115</i>: <br>
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<li>Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.
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<i>PSB1C3+E0240+J23116</i>: <br>
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Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.
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<br>
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<i>PSB1C3+E0240+J23117</i>: <br>  
<i>PSB1C3+E0240+J23117</i>: <br>  
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pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.<br>
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Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.<br>
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<br>
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<span class="cd-date">Sep 18</span>  
<span class="cd-date">Sep 18</span>  

Revision as of 19:57, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
PLAN DE PLAQUE


Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23102:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23104:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23105:
  • glycerol stock preparation from LB culture from the 17th September.
  • Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.

PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture from the 17th September.

PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.

PSB1C3+E0240+J23115:
  • Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.

    PSB1C3+E0240+J23116:
    Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.

    PSB1C3+E0240+J23117:
    Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.

    Sep 18

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