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Team:Evry/Interlab Study/09-11-2014
From 2014.igem.org
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<h2>Other constructions of the Anderson library of constitutive promoters</h2> | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
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| - | PSB1C3+E0240+J23101, <i>PSB1C3+E0240+J23118</i>, <i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>: | + | <i>PSB1C3+E0240+J23101</i>, <i>PSB1C3+E0240+J23118</i>, <i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>: |
<ul> | <ul> | ||
<li> Transformation plate from the 10th September observation. There were 50 colonies for each constructions. | <li> Transformation plate from the 10th September observation. There were 50 colonies for each constructions. | ||
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| + | <i>PSB1C3+E0240+J23101</i> and <i>PSB1C3+E0240+J23118</i>,<br> | ||
<li> PCR colony of 8 colonies for each construction following protocol table 1 and 2. | <li> PCR colony of 8 colonies for each construction following protocol table 1 and 2. | ||
https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg | https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg | ||
Revision as of 18:15, 19 September 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
- Transformation plate from the 10th September observation. There were 50 colonies for each constructions.
PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118,
- PCR colony of 8 colonies for each construction following protocol table 1 and 2.
https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
GEL 1: 1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. 
GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction. 
GEL 3: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction. - PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Preparation of samples to sequencing. N° XX
- Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
Sep 11
