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Team:Evry/Interlab Study/08-29-2014
From 2014.igem.org
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Transformation in DH5 alpha chemocompetent <i>E. coli</i> as doing on 21th August, with ampicilline LB agar plates | Transformation in DH5 alpha chemocompetent <i>E. coli</i> as doing on 21th August, with ampicilline LB agar plates | ||
<br/> Incubation overnight at 37°C. | <br/> Incubation overnight at 37°C. | ||
Revision as of 16:51, 19 September 2014
Construction n°1: PSB1C3 with I20260
Selected colonies PCR products from the 28/08/2014 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Colony n°2: 30.7 ng/µl and A260/280 1.92
- Colony n°4: 52.3 ng/µl and A260/280 1.75
- Colony n°7: 26.1 ng/µL and A260/280 1.72
Samples were send to sequencing. N° 26DJ96, 26DJ97, 26DJ98, 26DJ99, 26EA00 and 26EA01.
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
Transformation in DH5 alpha chemocompetent E. coli as doing on 21th August, with ampicilline LB agar plates
Incubation overnight at 37°C.
Aug 29
