Team:Evry/Interlab Study/08-29-2014

From 2014.igem.org

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<br/> Incubation overnight at 37°C.
<br/> Incubation overnight at 37°C.
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<span class="cd-date">Aug 29</span>  
<span class="cd-date">Aug 29</span>  

Revision as of 18:38, 17 September 2014

Picture

Construction n°1: PSB1C3 with I20260


Selected colonies PCR products from the 28/08/2014 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
  • Colony n°2: 30.7 ng/µl and A260/280 1.92
  • Colony n°4: 52.3 ng/µl and A260/280 1.75
  • Colony n°7: 26.1 ng/µL and A260/280 1.72

Samples were send to sequencing. N° 26DJ96, 26DJ97, 26DJ98, 26DJ99, 26EA00 and 26EA01.

Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Constructions with other promoters

Transformation in DH5 alpha chemocompetent E. coli as doing on 21th August, with ampicilline LB agar plates
Incubation overnight at 37°C.

Aug 29

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