From 2014.igem.org
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| | <h1>2.Why AMT？</h1> | | <h1>2.Why AMT？</h1> |
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| | + | Agrobacterium-mediated fungal transformation(AMT)is a potential tool for performing targeted and random mutagenesis .This method is commonly used for plant-cell transformations and recently widely applied to various fungus. In the AMT system,A.Tumefaciens is able to transfer T-DNA to a wide variety of fungi ,and it has especial high efficiency.</li><li> |
| | + | The gram-negative bacterium A. tumefaciens is a plant pathogen, which causes crown gall tumors.A. tumefaciens induces this tumorous growth by transferring a part of its DNA (T-DNA) ,which is located on its 200-kbp tumor-inducing (Ti) plasmid to the host. After integration into the host genome, genes that are naturally located on this T-DNA and encode enzymes for the production of metabolites and regulators for the plant growth. But another segment,the virulence region, which is composed of a large number of vir genes,is necessary for the tumorigenicity .So the binary vector system is used,in which the T-DNA and the virulence region are placed on two separate plasmids. |
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Revision as of 14:10, 17 September 2014
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1.The high-efficiency expression host—
Aspergillus niger is coming! |
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Aspergillus Niger is an important fermentation microbial ,which is widely used in industrial enzymes'production and organic acid fermentation.It can produce more than 30 kinds of enzyme product such as amylase, acid protease, cellulase, pectinase and glucose oxidase. In fact, Aspergillus Niger has been applied in the field of food production for a long time.Because of its excellent ability of secreting proteins, Aspergillus Niger is developed for universal heterologous protein expression host.
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As receptor bacteria of genetic engineering ,Aspergillus Niger has unique advantages upon bacteria and yeast .It's able to do majority kinds of processing after translation , and the glycosylation system is similar to that of higher eukaryotes.It's well known for high protein secretion capacity and recognized as the safe strain for heterologous protein production,in that the fermentation and post-processing technologies are mature.In addition,its genome sequences has already been published.
2.Why AMT？
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Agrobacterium-mediated fungal transformation(AMT)is a potential tool for performing targeted and random mutagenesis .This method is commonly used for plant-cell transformations and recently widely applied to various fungus. In the AMT system,A.Tumefaciens is able to transfer T-DNA to a wide variety of fungi ,and it has especial high efficiency.
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The gram-negative bacterium A. tumefaciens is a plant pathogen, which causes crown gall tumors.A. tumefaciens induces this tumorous growth by transferring a part of its DNA (T-DNA) ,which is located on its 200-kbp tumor-inducing (Ti) plasmid to the host. After integration into the host genome, genes that are naturally located on this T-DNA and encode enzymes for the production of metabolites and regulators for the plant growth. But another segment,the virulence region, which is composed of a large number of vir genes,is necessary for the tumorigenicity .So the binary vector system is used,in which the T-DNA and the virulence region are placed on two separate plasmids.
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