Team:Northwestern/Protocols
From 2014.igem.org
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<li><a href="#2" role="tab" data-toggle="tab">Ligation and Transformation</a></li> | <li><a href="#2" role="tab" data-toggle="tab">Ligation and Transformation</a></li> | ||
<li><a href="#3" role="tab" data-toggle="tab">Gel extraction</a></li> | <li><a href="#3" role="tab" data-toggle="tab">Gel extraction</a></li> | ||
- | <li><a href="#4" role="tab" data-toggle="tab"> | + | <li><a href="#4" role="tab" data-toggle="tab">Making Plates</a></li> |
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Revision as of 16:33, 16 September 2014
Protocols
![](https://static.igem.org/mediawiki/2014/c/cb/Flowchart_of_Cutting_DNA.jpg)
Note: dephosphorylation with Antarctic phophatase done after digestion. Can use CutSmart for dephosphorylation.
![](https://static.igem.org/mediawiki/2014/3/37/Flowchart_of_Gel_Extraction.png)
![](https://static.igem.org/mediawiki/2014/c/c2/Flowchart_of_making_plates.png)