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Team:Northwestern/Protocols
From 2014.igem.org
(Difference between revisions)
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<div class="container"> | <div class="container"> | ||
<ul class="nav nav-tabs" role="tablist"> | <ul class="nav nav-tabs" role="tablist"> | ||
| - | <li class="active"><a href="# | + | <li class="active"><a href="#1" role="tab" data-toggle="tab">Digestion</a></li> |
| - | <li><a href="# | + | <li><a href="#2" role="tab" data-toggle="tab">Ligation and Transformation</a></li> |
| - | <li><a href="# | + | <li><a href="#3" role="tab" data-toggle="tab">Gel extraction</a></li> |
| - | <li><a href="# | + | <li><a href="#4" role="tab" data-toggle="tab">PCR</a></li> |
</ul> | </ul> | ||
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<div class="tab-content"> | <div class="tab-content"> | ||
| - | <div class="tab-pane active" id=" | + | <div class="tab-pane active" id="1"> |
| - | <img src="https://static.igem.org/mediawiki/2014/c/cb/Flowchart_of_Cutting_DNA.jpg | + | <img src="https://static.igem.org/mediawiki/2014/c/cb/Flowchart_of_Cutting_DNA.jpg"/> |
<p>Note: dephosphorylation with Antarctic phophatase done <i>after</i> digestion. Can use CutSmart for dephosphorylation. </p> | <p>Note: dephosphorylation with Antarctic phophatase done <i>after</i> digestion. Can use CutSmart for dephosphorylation. </p> | ||
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| - | <div class="tab-pane" id=" | + | <div class="tab-pane" id="2"> |
| - | <img src="https://static.igem.org/mediawiki/2014/4/4d/IMG_5355.jpg | + | <img src="https://static.igem.org/mediawiki/2014/4/4d/IMG_5355.jpg"/> |
<p>for making competent cells to transform into</p> | <p>for making competent cells to transform into</p> | ||
<p>we used the following protocol</p> | <p>we used the following protocol</p> | ||
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| - | <div class="tab-pane" id=" | + | <div class="tab-pane" id="3"> |
| + | <img src="https://static.igem.org/mediawiki/2014/3/37/Flowchart_of_Gel_Extraction.png"/> | ||
| - | + | </div> | |
| - | <div class="tab-pane" id=" | + | <div class="tab-pane" id="4">PCR conditions we used, Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores. |
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.</div> | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.</div> | ||
Revision as of 16:30, 16 September 2014
Protocols
Note: dephosphorylation with Antarctic phophatase done after digestion. Can use CutSmart for dephosphorylation.
PCR conditions we used, Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
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