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Team:Northwestern/Protocols
From 2014.igem.org
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<img src="https://static.igem.org/mediawiki/2014/4/4d/IMG_5355.jpg" style="width: 100%;"/> | <img src="https://static.igem.org/mediawiki/2014/4/4d/IMG_5355.jpg" style="width: 100%;"/> | ||
| + | <p>for making competent cells to transform into</p> | ||
| + | <p>we used the following protocol</p> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3a/Protocols.jpg">raybiotech</a> | ||
</div> | </div> | ||
Revision as of 16:16, 16 September 2014
Protocols
Note: dephosphorylation with Antarctic phophatase done after digestion. Can use CutSmart for dephosphorylation.
Asking permission to post cell free protocols here Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
PCR conditions we used, Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
