Team:Northwestern/Protocols

From 2014.igem.org

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      <h1>Protocols</h1>
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Note: dephosphorylation with Antarctic phophatase done <i>after</i> digestion. Can use CutSmart for dephosphorylation.  
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<img src="https://static.igem.org/mediawiki/2014/c/cb/Flowchart_of_Cutting_DNA.jpg" style="width: 100%"/>
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<p>Note: dephosphorylation with Antarctic phophatase done <i>after</i> digestion. Can use CutSmart for dephosphorylation. </p>
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Revision as of 15:58, 16 September 2014

Dropdown menu from bootstrap


Protocols

Note: dephosphorylation with Antarctic phophatase done after digestion. Can use CutSmart for dephosphorylation.

Culturing S. rimosus, N, europaea, etc Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.

Asking permission to post cell free protocols here Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.
PCR conditions we used, Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quae repudiandae fugiat illo cupiditate excepturi esse officiis consectetur, laudantium qui voluptatem. Ad necessitatibus velit, accusantium expedita debitis impedit rerum totam id. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Natus quibusdam recusandae illum, nesciunt, architecto, saepe facere, voluptas eum incidunt dolores magni itaque autem neque velit in. At quia quaerat asperiores.