"

From 2014.igem.org

Revision as of 17:46, 15 September 2014 (view source) Acelentano (Talk | contribs) ← Older edit		Revision as of 20:46, 17 September 2014 (view source) Lenaw (Talk | contribs) Newer edit →
Line 100:		Line 100:
	<h1><center>The Sensor</center> </h1>		<h1><center>The Sensor</center> </h1>
-	<p> <center>Our sensor... Describe the process of putting together the sensor over the course of the summer...</center></p>	+	<p> <center>1. Fluorescent Protein Analysis</center></p>
		+	<p> <center>From our analysis of possible fluorescent protein reporters, we selected yellow fluorescent protein (YFP) and red fluorescent protein (RFP)</center></p>
		+	<p> <center>2. Construction: Overlap PCR</center></p>
		+	<p> <center>We first ligated the T7 promoter and RFP into the pSB3K3 plasmid, to create the second plasmid part of our sensor</center></p>
	<hr>		<hr>
	<center> <h1>Notebook Entries </h1></center>		<center> <h1>Notebook Entries </h1></center>

---

Revision as of 20:46, 17 September 2014

The Sensor

1. Fluorescent Protein Analysis

From our analysis of possible fluorescent protein reporters, we selected yellow fluorescent protein (YFP) and red fluorescent protein (RFP)

2. Construction: Overlap PCR

We first ligated the T7 promoter and RFP into the pSB3K3 plasmid, to create the second plasmid part of our sensor

---

Notebook Entries

Week One Week Two Week Three Week Four Week Five