Team:Evry/Notebook/Transformation/09-08-2014

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Revision as of 18:10, 11 September 2014

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
We made a restriction control of our plasmid, and we tried to prove that these plasmids are present in our Pseudovibrio.
After made a purification of plasmids in A1 and A2 with NucleoSpin Plamsid Kit (Macherey Nagel), we obtained concentrations at 380.1 ng/µL for pRhokHI-2 and at 91.1 ng/µL for pBBR1MCS.

We disgested these sample and our stock of pRhokHI-2 and pBBR1MCS with XbaI (unique restriction site on these plasmids) and we have make migrate the product of digestion.

Gel of digestion with XbaI

There was a problem with the digestion. We are going to try with a longer time of incubation.

Sep 08

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-	There was a problem with the digestion. We are going to try with ~~another enzyme~~.	+	There was a problem with the digestion. We are going to try with a longer time of incubation.