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Team:NCTU Formosa/biobricks
From 2014.igem.org
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======PBAN1====== | ======PBAN1====== | ||
*pcya and ho1 are the enzymes needed to convert heme into chromophore phycocyanobiline(PCB), the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use P<sub>cons</sub> upstream in order to make E.coli continuously expressed them to synthesize PCB. | *pcya and ho1 are the enzymes needed to convert heme into chromophore phycocyanobiline(PCB), the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use P<sub>cons</sub> upstream in order to make E.coli continuously expressed them to synthesize PCB. | ||
| - | <br>[[File:NCTU_Formosa_2014_Biobrick1|450px]] | + | <br>[[File:NCTU_Formosa_2014_Biobrick1.jpg|450px]] |
======PBAN2====== | ======PBAN2====== | ||
*Cph8 is a chimeric light receptor. It is a fusion of the photoreceptor cph1 and the envZ histidine kinase. cph1 is only active when it binds the chromophore phycocyanobiline (PCB). | *Cph8 is a chimeric light receptor. It is a fusion of the photoreceptor cph1 and the envZ histidine kinase. cph1 is only active when it binds the chromophore phycocyanobiline (PCB). | ||
| - | <br>[[File:NCTU_Formosa_2014_Biobrick2|150px]] | + | <br>[[File:NCTU_Formosa_2014_Biobrick2.jpg|150px]] |
======PBAN3====== | ======PBAN3====== | ||
*This is the key part to change Ompc promoter intro red promotor. The lacI will inhibit the function of P<sub>lac</sub>, thus, the original outcomes will be converted into our expected result. | *This is the key part to change Ompc promoter intro red promotor. The lacI will inhibit the function of P<sub>lac</sub>, thus, the original outcomes will be converted into our expected result. | ||
| - | <br>[[File:NCTU_Formosa_2014_Biobrick3|300px]] | + | <br>[[File:NCTU_Formosa_2014_Biobrick3.jpg|300px]] |
======PBAN4====== | ======PBAN4====== | ||
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======PBAN5====== | ======PBAN5====== | ||
| - | <br>[[File:NCTU_Formosa_2014_Biobrick5|200px]] | + | <br>[[File:NCTU_Formosa_2014_Biobrick5.jpg|200px]] |
======PBAN6====== | ======PBAN6====== | ||
*By using mGFP as a reporter gene, we can test whether the 37 °C RBS works. | *By using mGFP as a reporter gene, we can test whether the 37 °C RBS works. | ||
| - | <br>[[File:NCTU_Formosa_2014_Biobrick6|200px]] | + | <br>[[File:NCTU_Formosa_2014_Biobrick6.jpg|200px]] |
======PBAN7====== | ======PBAN7====== | ||
*In our circuit, this biobrick is the part of P<sub>lux</sub>'s activation when the temperature reaches to 37<sup>o</sup>C. | *In our circuit, this biobrick is the part of P<sub>lux</sub>'s activation when the temperature reaches to 37<sup>o</sup>C. | ||
<br> | <br> | ||
| - | [[File:NCTU_Formosa_2014_Biobrick7|200px]] | + | [[File:NCTU_Formosa_2014_Biobrick7.jpg|200px]] |
======PBAN8====== | ======PBAN8====== | ||
*The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work. In addition, adding Plux upstream makes the sequence be controlled by luxR/AHL complex.<br> | *The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work. In addition, adding Plux upstream makes the sequence be controlled by luxR/AHL complex.<br> | ||
| - | [[File:NCTU_Formosa_2014_Biobrick8|250px]] | + | [[File:NCTU_Formosa_2014_Biobrick8.jpg|250px]] |
======PBAN9====== | ======PBAN9====== | ||
*The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work. | *The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work. | ||
| - | <br>[[File:NCTU_Formosa_2014_Biobrick9|200px]] | + | <br>[[File:NCTU_Formosa_2014_Biobrick9.jpg|200px]] |
Revision as of 11:20, 10 September 2014
Project
Parts submitted to the Registry
<groupparts>iGEM014 NCTU_Formosa</groupparts>
Brief Information
Please click on the name of the parts for detailed information that is hosted in the Registry website.
PBAN-producted system
PBAN1
- pcya and ho1 are the enzymes needed to convert heme into chromophore phycocyanobiline(PCB), the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use Pcons upstream in order to make E.coli continuously expressed them to synthesize PCB.
PBAN2
- Cph8 is a chimeric light receptor. It is a fusion of the photoreceptor cph1 and the envZ histidine kinase. cph1 is only active when it binds the chromophore phycocyanobiline (PCB).
PBAN3
- This is the key part to change Ompc promoter intro red promotor. The lacI will inhibit the function of Plac, thus, the original outcomes will be converted into our expected result.
PBAN4
- Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark.
PBAN5
PBAN6
- By using mGFP as a reporter gene, we can test whether the 37 °C RBS works.
PBAN7
- In our circuit, this biobrick is the part of Plux's activation when the temperature reaches to 37oC.
PBAN8
- The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work. In addition, adding Plux upstream makes the sequence be controlled by luxR/AHL complex.
PBAN9
- The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work.
