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Team:Kyoto/Notebook/DMS Synthesis
From 2014.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
<h2>7/31</h2> | <h2>7/31</h2> | ||
<h3>Restriction Enzyme Digestion</h3> | <h3>Restriction Enzyme Digestion</h3> | ||
| - | <span class="author"> | + | <span class="author">Nakamura Matsumoto</span> |
<table class="note"> | <table class="note"> | ||
<tr><th>Sample</th><th>Volume</th><th>EcoR1</th><th>Spe1</th><th>Buffur</th><th>MilliQ</th><th>TOTAL</tr> | <tr><th>Sample</th><th>Volume</th><th>EcoR1</th><th>Spe1</th><th>Buffur</th><th>MilliQ</th><th>TOTAL</tr> | ||
| Line 20: | Line 20: | ||
<h2>8/31</h2> | <h2>8/31</h2> | ||
<h3>Gel Extraction</h3> | <h3>Gel Extraction</h3> | ||
| - | <span class="author"> | + | <span class="author">Yasuda Murata</span> |
<table class="note"> | <table class="note"> | ||
<tr><th>Lane</th><th>DNA</th> | <tr><th>Lane</th><th>DNA</th> | ||
| Line 33: | Line 33: | ||
<tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr> | <tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr> | ||
<tr><td>10</td><td>pSB1C3(control)</td></tr> | <tr><td>10</td><td>pSB1C3(control)</td></tr> | ||
| + | |||
| + | <h2>8/3</h2> | ||
| + | <h3>Electrophoresis for confirmation</h3> | ||
| + | <span class="author">Murata</span> | ||
| + | <table class="note"> | ||
| + | <tr><th>Lane</th><th>Sample</th></tr> | ||
| + | <tr><td>1</td><td>pSB1C3 dddD 1</td></tr> | ||
| + | <tr><td>2</td><td>pSB1C3 dddD 2</td></tr> | ||
| + | <tr><td>3</td><td>pSB1C3 dddD 3</td></tr> | ||
| + | <tr><td>4</td><td>1kb ladder(3μL)</td></tr> | ||
| + | <tr><td>5</td><td>1kb ladder(1μL)</td></tr> | ||
| + | </table> | ||
Revision as of 02:25, 10 September 2014
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アメフラシノート
7/31
Restriction Enzyme Digestion
| Sample | Volume | EcoR1 | Spe1 | Buffur | MilliQ | TOTAL |
|---|---|---|---|---|---|---|
| 3/24 PCR(dddD)cloning | 3.2 | 1 | 1 | 3(H) | 21.8 | 30 |
| pSB1C3 | 18.5 | 1 | 1 | 3(H) | 6.5 | 30 |
| pSB1C3(control) | 6.2 | 0 | 0 | 3(H) | 2.8 | 10 |
8/31
Gel Extraction
| Lane | DNA |
|---|---|
| 1 | 1kbp ladder |
| 2 | 3/24 PCR(dddD)cloning E,S |
| 3 | 3/24 PCR(dddD)cloning E,S |
| 4 | Blank |
| 5 | pSB1C3 |
| 6 | pSB1C3 |
| 7 | Blank |
| 8 | 1kbp ladder |
| 9 | 3/24 PCR(dddD)cloning(control) |
| 10 | pSB1C3(control) |
| Lane | Sample |
|---|---|
| 1 | pSB1C3 dddD 1 |
| 2 | pSB1C3 dddD 2 |
| 3 | pSB1C3 dddD 3 |
| 4 | 1kb ladder(3μL) |
| 5 | 1kb ladder(1μL) |
