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Team:Kyoto/Notebook/DMS Synthesis
From 2014.igem.org
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<table class="note"> | <table class="note"> | ||
<tr><th>Lane</th><th>DNA</th> | <tr><th>Lane</th><th>DNA</th> | ||
| - | <tr><td>1</td><td> | + | <tr><td>1</td><td>1kbp ladder</td></tr> |
| - | <tr><td>2</td><td> | + | <tr><td>2</td><td>3/24 PCR(dddD)cloning E,S</td></tr> |
| - | <tr><td>3</td><td> | + | <tr><td>3</td><td>3/24 PCR(dddD)cloning E,S</td></tr> |
| - | <tr><td>4</td><td> | + | <tr><td>4</td><td>Blank</td></tr> |
| - | <tr><td>5</td><td> | + | <tr><td>5</td><td>pSB1C3</td></tr> |
| - | <tr><td>6</td><td> | + | <tr><td>6</td><td>pSB1C3</td></tr> |
| - | <tr><td>7</td><td> | + | <tr><td>7</td><td>Blank</td></tr> |
| - | <tr><td>8</td><td> | + | <tr><td>8</td><td>1kbp ladder</td></tr> |
| - | <tr><td>9</td><td> | + | <tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr> |
| - | <tr><td>10</td><td> | + | <tr><td>10</td><td>pSB1C3(control)</td></tr> |
</table> | </table> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 01:41, 10 September 2014
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アメフラシノート
7/31
Restriction Enzyme Digestion
| Sample | Volume | EcoR1 | Spe1 | Buffur | MilliQ | TOTAL |
|---|---|---|---|---|---|---|
| 3/24 PCR(dddD)cloning | 3.2 | 1 | 1 | 3(H) | 21.8 | 30 |
| pSB1C3 | 18.5 | 1 | 1 | 3(H) | 6.5 | 30 |
| pSB1C3(control) | 6.2 | 0 | 0 | 3(H) | 2.8 | 10 |
8/31
Gel Extraction
| Lane | DNA |
|---|---|
| 1 | 1kbp ladder |
| 2 | 3/24 PCR(dddD)cloning E,S |
| 3 | 3/24 PCR(dddD)cloning E,S |
| 4 | Blank |
| 5 | pSB1C3 |
| 6 | pSB1C3 |
| 7 | Blank |
| 8 | 1kbp ladder |
| 9 | 3/24 PCR(dddD)cloning(control) |
| 10 | pSB1C3(control) |
