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STREAM/3 June 2014
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'''June 3, 2014''' | '''June 3, 2014''' | ||
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Purpose: To isolate plasmid DNA from MACH cells | Purpose: To isolate plasmid DNA from MACH cells | ||
Protocol from GeneJet Plasmid Miniprep kit: http://www.thermoscientificbio.com/uploadedfiles/resources/k0502-product-information.pdf | Protocol from GeneJet Plasmid Miniprep kit: http://www.thermoscientificbio.com/uploadedfiles/resources/k0502-product-information.pdf | ||
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''TECAN'' | ''TECAN'' | ||
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Purpose: To measure fluorescence from MACH Cells | Purpose: To measure fluorescence from MACH Cells | ||
Latest revision as of 16:51, 9 September 2014
June 3, 2014
MiniPreps of MACH Cells Expressing Fluorescence
Purpose: To isolate plasmid DNA from MACH cells
Protocol from GeneJet Plasmid Miniprep kit: http://www.thermoscientificbio.com/uploadedfiles/resources/k0502-product-information.pdf
TECAN
Purpose: To measure fluorescence from MACH Cells
Cell Cultures of MACH Cells for Fluorescence: 3 mL LB and 300 μL cell culture at 10:45 AM Time point 1: 12:45 PM Time point 2: 4:45 PM
To analyze fluorescence at given time points
In Row D: 50 μL cell culture (at time point 1)
Used the following parameters to observe the gain and brightness of the following fluorescent proteins:
