Team:UT-Dallas/Notebook/8-25

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<h1 class="firstHeading" align="center">Monday, August 25th, 2014</h1>
<h1 class="firstHeading" align="center">Monday, August 25th, 2014</h1>

Revision as of 02:54, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Monday, August 25th, 2014

Click here to edit the page

On the weekend, we did a bunch of inoculation and minipreps reporter vectors and gRNAs. Today we test digest them. If they're all good, they're good to go for sequencing and the last thing to do would be almost just waiting for cas9 to be delivered. Classes started again for everyone except Sam and Tyler.

Today's tasks:
  • Test digested Carb reporter vectors: tcpB, tcpQ, tcpR, tcpT, acfC, acfD, tcpE, tcpF
  • Test digested gRNA vectors: acfA, acfB, tcpA, tcpF, tcpQ
  • Test digested & gel purified Chlora-reporter vectors for acfA.


Test digest was great! Successfully built all the gRNA on Chlora!


Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-25"