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Team:BYU Provo/Notebook/Metabolism/septoct
From 2014.igem.org
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<h2>Week of September 6th</h2> | <h2>Week of September 6th</h2> | ||
<h3>September 3, 2014</h3> | <h3>September 3, 2014</h3> | ||
| - | <p>--CS-- Today I checked the sequencing results from all of last week's sequencing. The samples that I submitted for <i>nirS</i> (3-5) and <i>norC</i> (3-2 and 3-4) all turned out great! The results showed that the promoters were inserted into the plasmids upstream of the gene sequences, just as we wanted them. I also checked the <i>norB</i> mutagenesis results... | + | <p>--CS-- Today I checked the sequencing results from all of last week's sequencing. The samples that I submitted for <i>nirS</i> (3-5) and <i>norC</i> (3-2 and 3-4) all turned out great! The results showed that the promoters were inserted into the plasmids upstream of the gene sequences, just as we wanted them. I also checked the <i>norB</i> mutagenesis results. It appears that they did not work properly, so we'll have to try those all over again. The PCR hasn't been working properly, so I think I should try starting over from there instead of transforming and everything all over again. Julie and I also talked and decided that Julie would take over with constructing the ginormous plasmid containing all four of the denitrification genes since she is pretty much done with her antibiotics stuff now.</p> |
| - | </p> | + | |
| + | <h2>Week of September 13th</h2> | ||
| + | <h3>September 8, 2014</h3> | ||
| + | <p>--CS-- Today I reviewed where everything is at right now with the denitrification project. I <a href="https://2014.igem.org/Team:BYU_Provo/Notebook/CommonProcedures">transformed</a> 2 μl and 4 μl of the <i>DpnI</i>-digested <i>nosZ</i> into DH5α, letting it incubate at 37°C for 1.5 hours, and then plated it onto LB+Cam plates, putting them in the old 37°C incubator overnight. </p> | ||
</body> | </body> | ||
Revision as of 02:08, 9 September 2014
BYU 2014 Notebook |
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Week of September 6th
September 3, 2014
--CS-- Today I checked the sequencing results from all of last week's sequencing. The samples that I submitted for nirS (3-5) and norC (3-2 and 3-4) all turned out great! The results showed that the promoters were inserted into the plasmids upstream of the gene sequences, just as we wanted them. I also checked the norB mutagenesis results. It appears that they did not work properly, so we'll have to try those all over again. The PCR hasn't been working properly, so I think I should try starting over from there instead of transforming and everything all over again. Julie and I also talked and decided that Julie would take over with constructing the ginormous plasmid containing all four of the denitrification genes since she is pretty much done with her antibiotics stuff now.
Week of September 13th
September 8, 2014
--CS-- Today I reviewed where everything is at right now with the denitrification project. I transformed 2 μl and 4 μl of the DpnI-digested nosZ into DH5α, letting it incubate at 37°C for 1.5 hours, and then plated it onto LB+Cam plates, putting them in the old 37°C incubator overnight.
