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		+	<h2 style="003468">Week of September 5th</h2>
		+	<h3>Sept 3</h3>
		+	<p> (TR,CB) Performed SOEing PCR on the front and back halves of insert to go into pSR47s plasmid.  We used the high fidelity q5 DNA polymerase.</p>
		+	<h3>Sept 4</h3>
		+	<p> (TR) Ran our PCR product from yesterday on a gel electrophoresis to determine if the SOEing worked.  The gel came out with only a band at the 500bp range, therefore we know it didn't work.  This shouldn't be a problem since we are still waiting to have some <i>N. multiformis</i> grow to be used in conjugation.</p>
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Revision as of 00:43, 23 September 2014

BYU 2014 Notebook Edit September October

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Week of September 5th

Sept 3

(TR,CB) Performed SOEing PCR on the front and back halves of insert to go into pSR47s plasmid. We used the high fidelity q5 DNA polymerase.

Sept 4

(TR) Ran our PCR product from yesterday on a gel electrophoresis to determine if the SOEing worked. The gel came out with only a band at the 500bp range, therefore we know it didn't work. This shouldn't be a problem since we are still waiting to have some N. multiformis grow to be used in conjugation.