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Team:Sumbawagen/Notebook
From 2014.igem.org
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<p>We began our project by preparing material needed. We made liquid and solid medium using LB powder. We also made solution for transformation process like MgCl2, and TSS medium. In this week, we also culture the stock of our RFP in liquid medium. We aim to make the stock of RFP and also examine whether the RFP still work or not. The culture of RFP is not growth. It indicates that our RFP stock has been damaged due to the unstable storage temperature. </p> | <p>We began our project by preparing material needed. We made liquid and solid medium using LB powder. We also made solution for transformation process like MgCl2, and TSS medium. In this week, we also culture the stock of our RFP in liquid medium. We aim to make the stock of RFP and also examine whether the RFP still work or not. The culture of RFP is not growth. It indicates that our RFP stock has been damaged due to the unstable storage temperature. </p> | ||
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| + | <strong>Detail Activity: </strong> | ||
| + | <p>1.August 14 th, 2014</p> | ||
| + | Making liquid medium using LB medium powder, sterilization using autoclave, add antibiotic, culture E coli DH5α, shaking | ||
| + | <p>2.August 15th, 2014</p> | ||
| + | Making solution MgSO4, glucose 1 M, CaCl2, TSS medium | ||
| + | <p>3.August 16th, 2014</p> | ||
| + | Making solid, liquid, and NA medium, Culture E coli (Glycerol stock RFP) | ||
| + | <p>4.August 17th, 2014</p> | ||
| + | Add chlorampenichol to solid medium (LB medium), streak RFP (stock 6 tubes), check culture E coli (Glycerol stock RFP) | ||
<h2>Week 2</h2> | <h2>Week 2</h2> | ||
Revision as of 02:03, 8 September 2014
Team:Sumbawagen/Team
From 2014.igem.org
Week 1
We began our project by preparing material needed. We made liquid and solid medium using LB powder. We also made solution for transformation process like MgCl2, and TSS medium. In this week, we also culture the stock of our RFP in liquid medium. We aim to make the stock of RFP and also examine whether the RFP still work or not. The culture of RFP is not growth. It indicates that our RFP stock has been damaged due to the unstable storage temperature.
Detail Activity:1.August 14 th, 2014
Making liquid medium using LB medium powder, sterilization using autoclave, add antibiotic, culture E coli DH5α, shaking2.August 15th, 2014
Making solution MgSO4, glucose 1 M, CaCl2, TSS medium3.August 16th, 2014
Making solid, liquid, and NA medium, Culture E coli (Glycerol stock RFP)4.August 17th, 2014
Add chlorampenichol to solid medium (LB medium), streak RFP (stock 6 tubes), check culture E coli (Glycerol stock RFP)Week 2
In the second week we culture the stock of RFP into the solid medium. The purpose is to make the stock of RFP in the solid medium. We also transformed the plasmid Bba_J04450 and Bba_B0034 into E coli BL21 (DE3). We did transformation twice in this week. The first transformation does not show any red colony growing. But the second transformation showed red colonies growing indicates that the transformation process success.
Detail Activity :
1. August 18th, 2014
Making solid medium with chlorampenicol, take Bba_B0034 from kit plate and storage2. August 19th, 2014
Making ampicilin stock 1 M, streak RFP (stock lab) medium padat, transformation3. August 20th, 2014
Check Bba_J04450 transformation result, culture RFP, streak RFP, making ampicilin stock 1 M, plating transformation result, streak RFP from solid medium4. August 21th, 2014
Check transformation results (Not growing), check RFP streak (contaminant), streak transformation result5. August 22th, 2014
Culture E coli glycerol stock (BL21(DE3)), Transformation
Week 3
Detail Activity
1. 25 August, 2014
Culture RFP DH5α (Add over expression reagent), making chlorampenicol stock2. 26 August, 2014
Culture E coli glycerol stock RFP, culture over expression DH5α in 6 tube, streak RFP from single colony and line colony to the LB medium with and without chlorampenicol3. 27 August, 2014
Plating transformation results, streak stock RFPSponsors
